@article{fdi:010083352,
  title = {{A} glutamate synthase mutant of {B}radyrhizobium sp. strain {ORS}285 is unable to induce nodules on {N}od factor-independent {A}eschynomene species},
  author = {{N}ouwen, {N}ico and {C}haintreuil, {C}l{\'e}mence and {F}ardoux, {J}o{\¨e}l and {G}iraud, {E}ric},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he {B}radyrhizobium sp. strain {ORS}285 is able to establish a nitrogen-fixing symbiosis with both {N}od factor ({NF}) dependent and {NF}-independent {A}eschynomene species. {H}ere, we have studied the growth characteristics and symbiotic interaction of a glutamate synthase ({GOGAT}; glt{D}::{T}n5) mutant of {B}radyrhizobium {ORS}285. {W}e show that the {ORS}285 glt{D}::{T}n5 mutant is unable to use ammonium, nitrate and many amino acids as nitrogen source for growth and is unable to fix nitrogen under free-living conditions. {M}oreover, on several nitrogen sources, the growth rate of the glt{B}::{T}n5 mutant was faster and/or the production of the carotenoid spirilloxanthin was much higher as compared to the wild-type strain. {T}he absence of {GOGAT} activity has a drastic impact on the symbiotic interaction with {NF}-independent {A}eschynomene species. {W}ith these species, inoculation with the {ORS}285 glt{D}::{T}n5 mutant does not result in the formation of nodules. {I}n contrast, the {ORS}285 glt{D}::{T}n5 mutant is capable to induce nodules on {NF}-dependent {A}eschynomene species, but these nodules were ineffective for nitrogen fixation. {I}nterestingly, in {NF}-dependent and {NF}-independent {A}eschynomene species inoculation with the {ORS}285 glt{D}::{T}n5 mutant results in browning of the plant tissue at the site of the infection suggesting that the mutant bacteria induce plant defence responses.},
  keywords = {},
  booktitle = {},
  journal = {{S}cientific {R}eports - {N}ature},
  volume = {11},
  numero = {1},
  pages = {20910 [11 ]},
  ISSN = {2045-2322},
  year = {2021},
  DOI = {10.1038/s41598-021-00480-7},
  URL = {https://www.documentation.ird.fr/hor/fdi:010083352},
}