@article{fdi:010083234,
  title = {{P}harmacological validation of {S}olanum mammosum {L}. as an anti-infective agent : role of solamargine},
  author = {{C}abanillas, {B}. and {C}hassagne, {F}ran{\c{c}}ois and {V}asquez-{O}cmin, {P}. and {T}ahrioui, {A}. and {C}hevalier, {S}. and {V}ansteelandt, {M}. and {T}riastuti, {A}. and {G}uerra, {C}. {A}. {A}. and {F}abre, {N}. and {H}addad, {M}ohamed},
  editor = {},
  language = {{ENG}},
  abstract = {{E}thnopharmacological relevance: {F}ungal and bacterial infections remain a major problem worldwide, requiring the development of effective therapeutic strategies. {S}olanum mammosum {L}. ({S}olanaceae) ("teta de vaca") is used in traditional medicine in {P}eru to treat fungal infections and respiratory disorders via topical application. {H}owever, the mechanism of action remains unknown, particularly in light of its chemical composition. {M}aterials and methods: {T}he antifungal activity of {TDV} was determined against {T}richophyton mentagrophytes and {C}andida albicans using bioautography-{TLC}-{HRMS} to rapidly identify the active compounds. {T}hen, the minimum inhibitory concentration ({MIC}) of the fruit crude extract and the active compound was determined to precisely evaluate the antifungal activity. {A}dditionally, the effects of the most active compound on the formation of {P}seudomonas aeruginosa biofilms and pyocyanin production were evaluated. {F}inally, a {LC}-{HRMS} profile and a molecular network of {TDV} extract were created to characterize the metabolites in the fruits' ethanolic extract. {R}esults: {B}ioautography-{TLC}-{HRMS} followed by isolation and confirmation of the structure of the active compound by 1{D} and 2{D} {NMR} allowed the identification solamargine as the main compound responsible for the anti{T}richophyton mentagrophytes ({MIC} = 64 mu g m{L}(-1)) and anti-{C}andida albicans ({MIC} = 64 mu g m{L}(-1)) activities. {I}n addition, solamargine led to a significant reduction of about 20% of the {P}seudomonas aeruginosa biofilm formation. {T}his effect was observed at a very low concentration (1.6 mu g m{L}(-1)) and remained fairly consistent regardless of the concentration. {I}n addition, solamargine reduced pyocyanin production by about 20% at concentrations of 12.5 and 50 mu g m{L}(-1). {F}urthermore, the {LC}-{HRMS} profiling of {TDV} allowed us to annotate seven known compounds that were analyzed through a molecular network. {C}onclusions: {S}olamargine has been shown to be the most active compound against {T}. mentoagrophytes and {C}. albicans in vitro. {I}n addition, our data show that this compound affects significantly {P}. aeruginosa pyocyanin production and biofilm formation in our conditions. {A}ltogether, these results might explain the traditional use of {S}. mammosum fruits to treat a variety of fungal infections and respiratory disorders.},
  keywords = {{S}olanum mammosum ; {C}andida albicans ; {T}richophyton mentagrophytes ; {P}seudomonas aeruginosa ; {B}ioautography ; {S}olamargine ; {LC}-{HRMS} ; {AMERIQUE} {DU} {SUD} ; {AMERIQUE} {CENTRALE} ; {PEROU} ; {ZONE} {TROPICALE} ; {AMAZONIE}},
  booktitle = {},
  journal = {{J}ournal of {E}thnopharmacology},
  volume = {280},
  numero = {},
  pages = {114473 [8 ]},
  ISSN = {0378-8741},
  year = {2021},
  DOI = {10.1016/j.jep.2021.114473},
  URL = {https://www.documentation.ird.fr/hor/fdi:010083234},
}