@article{fdi:010083060,
  title = {{C}hemodiversity of {C}alophyllum inophyllum {L}. oil bioactive components related to their specific geographical distribution in the {S}outh {P}acific region},
  author = {{G}inigini, {J}. and {L}ecellier, {G}.{J}. and {N}icolas, {M}. and {N}our, {M}. and {H}nawia, {E}douard and {L}ebouvier, {N}. and {H}erbette, {G}. and {L}ockhart, {P}. and {R}aharivelomanana, {P}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {D}ifferent parts of the tree {C}alophyllum inophyllum {L}. (nuts, leaves, roots, bark, fruits, nut oil and resin) are used as traditional medicines and cosmetics in most of the {P}acific {I}slands. {T}he oil efficiency as a natural cure and in traditional cosmetics has been largely described throughout the {S}outh {P}acific, which led us to investigate {C}. inophyllum's chemical and genetic diversity. {A} correlative study of the nut resin and leaf {DNA} from three distinct archipelagos in the {S}outh {P}acific was carried out in order to identify diversity patterns in {C}. inophyllum across the {S}outh {P}acific.



{M}ethods: {C}alophyllum inophyllum plants were sampled from {F}rench {P}olynesia, {N}ew {C}aledonia and {F}iji. {W}e extracted tamanu oil (nut oil) resin for chemo-diversity studies and sampled leaf tissues for genetic studies. {W}e applied an analysis method designed for small quantities (at a microscale level), and used {H}igh {P}erformance {L}iquid {C}hromatography ({HPLC}) to establish the chemo-diversity of tamanu oil resin. {I}n-house standards were co-eluted for qualitative determination. {G}enetic diversity was assessed using chloroplast barcoding markers (the {A}cetyl-{C}o{A} carboxylase (acc{D}) gene and the psa{A}-ycf3 intergenic spacer region).



{R}esults: {O}ur {HPLC} analysis revealed 11 previously known tamanu oil constituents, with variability among plant samples. {W}e also isolated and characterized two new neoflavonoids from tamanu oil resin namely, tamanolide {E}1 and {E}2 which are diastereoisomers. {A}lthough genetic analysis revealed low genetic variation, our multivariate analysis ({PCA}) of the tamanu oil resin chemical profiles revealed differentiation among geographic regions.



{C}onclusion: {W}e showed here that chromatographic analysis using formalized in-house standards of oil resin compounds for co-elution studies against oil resin samples could identify patterns of variation among samples of {C}. inophyllum, and discriminate samples from different geographical origins.},
  keywords = {{TRADITION} ; {PLANTE} {MEDICINALE} ; {BIOCHIMIE} ; {CHROMATOGRAPHIE} ; {EXTRACTION} ; {DIVERSITE} {GENETIQUE} ; {HUILE} {VEGETALE} ; {NEOFLAVONOIDE} ; {COSMETIQUE} ; {PACIFIQUE} {ILES} ; {POLYNESIE} {FRANCAISE} ; {NOUVELLE} {CALEDONIE} ; {FIDJI}},
  booktitle = {},
  journal = {{P}eer{J}},
  volume = {7},
  numero = {},
  pages = {e6896 [21 ]},
  ISSN = {2167-8359},
  year = {2019},
  DOI = {10.7717/peerj.6896},
  URL = {https://www.documentation.ird.fr/hor/fdi:010083060},
}