@article{fdi:010082406,
  title = {{C}omparing environmental {DNA} metabarcoding and underwater visual census to monitor tropical reef fishes},
  author = {{P}olanco {F}ernández, {A}. and {M}arques, {V}. and {F}opp, {F}. and {J}uhel, {J}.{B}. and {B}orrero-{P}{\'e}rez, {G}.{H}. and {C}heutin, {M}.{C}. and {D}ejean, {T}. and {G}onzález {C}orredor, {J}.{D}. and {A}costa-{C}haparro, {A}. and {H}ocd{\'e}, {R}{\'e}gis and {E}me, {D}. and {M}aire, {E}. and {S}pescha, {M}. and {V}alentini, {A}. and {M}anel, {S}. and {M}ouillot, {D}. and {A}lbouy, {C}. and {P}ellissier, {L}.},
  editor = {},
  language = {{ENG}},
  abstract = {{E}nvironmental {DNA} (e{DNA}) analysis is a revolutionary method to monitor marine biodiversity from animal {DNA} traces. {E}xamining the capacity of e{DNA} to provide accurate biodiversity measures in species-rich ecosystems such as coral reefs is a prerequisite for their application in long-term monitoring. {H}ere, we surveyed two {C}olombian tropical marine reefs, the island of {P}rovidencia and {G}ayraca {B}ay near {S}anta {M}arta, using e{DNA} and underwater visual census ({UVC}) methods. {W}e collected a large quantity of surface water (30 {L} per filter) above the reefs and applied a metabarcoding protocol using three different primer sets targeting the 12{S} mitochondrial {DNA}, which are specific to the vertebrates {A}ctinopterygii and {E}lasmobranchii. {B}y assigning e{DNA} sequences to species using a public reference database, we detected the presence of 107 and 85 fish species, 106 and 92 genera, and 73 and 57 families in {P}rovidencia and {G}ayraca {B}ay, respectively. {O}f the species identified using e{DNA}, 32.7/100 ({P}rovidencia) and 18.8/100 ({G}ayraca) were also found in the {UVC}s. {W}e further found congruence in genus and species richness and abundance between e{DNA} and {UVC} approaches in {P}rovidencia but not in {G}ayraca {B}ay. {M}ismatches between e{DNA} and {UVC} had a phylogenetic and ecological signal, with e{DNA} detecting a broader phylogenetic diversity and more effectively detecting smaller species, pelagic species and those in deeper habitats. {A}ltogether, e{DNA} can be used for fast and broad biodiversity surveys and is applicable to species-rich ecosystems in the tropics, but improved coverage of the reference database is required before this new method could serve as an effective complement to traditional census methods.},
  keywords = {{COLOMBIE} ; {SAN} {ANDRES} {Y} {PROVIDENCIA} ; {ZONE} {TROPICALE} ; {PARC} {NATIONAL} {DE} {TAYRONA} ; {CARAIBES} {MER} ; {BAIE} {DE} {GAYRACA}},
  booktitle = {{T}he future of biodiversity monitoring and conservation utilizing environmental {DNA}},
  journal = {{E}nvironmental {DNA}},
  volume = {3},
  numero = {1},
  pages = {142--156 [en ligne]},
  ISSN = {2637-4943},
  year = {2021},
  DOI = {10.1002/edn3.140},
  URL = {https://www.documentation.ird.fr/hor/fdi:010082406},
}