@article{fdi:010082306,
  title = {{ANCHOR} : a technical approach to monitor single-copy locus localization in planta},
  author = {{M}eschichi, {A}. and {I}ngouff, {M}. and {P}icart, {C}. and {M}irouze, {M}arie and {D}esset, {S}. and {G}allardo, {F}. and {B}ystricky, {K}. and {P}icault, {N}. and {R}osa, {S}. and {P}ontvianne, {F}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}ogether with local chromatin structure, gene accessibility, and the presence of transcription factors, gene positioning is implicated in gene expression regulation. {A}lthough the basic mechanisms are expected to be conserved in eukaryotes, less is known about the role of gene positioning in plant cells, mainly due to the lack of a highly resolutive approach. {I}n this study, we adapted the use of the {ANCHOR} system to perform real-time single locus detection in planta. {ANCHOR} is a {DNA}-labeling tool derived from the chromosome partitioning system found in many bacterial species. {W}e demonstrated its suitability to monitor a single locus in planta and used this approach to track chromatin mobility during cell differentiation in {A}rabidopsis thaliana root epidermal cells. {F}inally, we discussed the potential of this approach to investigate the role of gene positioning during transcription and {DNA} repair in plants.},
  keywords = {chromatin ; nuclear organization ; real-time imaging ; microscopy ; single-locus analysis ; chromatin mobility},
  booktitle = {},
  journal = {{F}rontiers in {P}lant {S}cience},
  volume = {12},
  numero = {},
  pages = {677849 [9 ]},
  ISSN = {1664-462{X}},
  year = {2021},
  DOI = {10.3389/fpls.2021.677849},
  URL = {https://www.documentation.ird.fr/hor/fdi:010082306},
}