Guissou E., Waite J. L., Jones M., Bell A. S., Suh E., Yameogo K. B., Djégbé N., Da D. F., Hien Dfds, Yerbanga R. S., Ouedraogo A. G., Dabiré K. R., Cohuet Anna, Thomas M. B., Lefèvre Thierry. (2021). A non-destructive sugar-feeding assay for parasite detection and estimating the extrinsic incubation period of Plasmodium falciparum in individual mosquito vectors. Scientific Reports - Nature, 11 (1), 9344 [14 p.]. ISSN 2045-2322.
Titre du document
A non-destructive sugar-feeding assay for parasite detection and estimating the extrinsic incubation period of Plasmodium falciparum in individual mosquito vectors
Année de publication
2021
Auteurs
Guissou E., Waite J. L., Jones M., Bell A. S., Suh E., Yameogo K. B., Djégbé N., Da D. F., Hien Dfds, Yerbanga R. S., Ouedraogo A. G., Dabiré K. R., Cohuet Anna, Thomas M. B., Lefèvre Thierry
Source
Scientific Reports - Nature, 2021,
11 (1), 9344 [14 p.] ISSN 2045-2322
Despite its epidemiological importance, the time Plasmodium parasites take to achieve development in the vector mosquito (the extrinsic incubation period, EIP) remains poorly characterized. A novel non-destructive assay designed to estimate EIP in single mosquitoes, and more broadly to study Plasmodium-Anopheles vectors interactions, is presented. The assay uses small pieces of cotton wool soaked in sugar solution to collect malaria sporozoites from individual mosquitoes during sugar feeding to monitor infection status over time. This technique has been tested across four natural malaria mosquito species of Africa and Asia, infected with Plasmodium falciparum (six field isolates from gametocyte-infected patients in Burkina Faso and the NF54 strain) and across a range of temperatures relevant to malaria transmission in field conditions. Monitoring individual infectious mosquitoes was feasible. The estimated median EIP of P. falciparum at 27 degrees C was 11 to 14 days depending on mosquito species and parasite isolate. Long-term individual tracking revealed that sporozoites transfer onto cotton wool can occur at least until day 40 post-infection. Short individual EIP were associated with short mosquito lifespan. Correlations between mosquito/parasite traits often reveal trade-offs and constraints and have important implications for understanding the evolution of parasite transmission strategies.
Plan de classement
Entomologie médicale / Parasitologie / Virologie [052]
Localisation
Fonds IRD [F B010082111]
Identifiant IRD
fdi:010082111
