@article{fdi:010081517,
  title = {{E}xploring the diversity of fungal {D}y{P}s in mangrove soils to produce and characterize novel biocatalysts},
  author = {{B}en {A}yed, {A}. and {S}aint-{G}enis, {G}. and {V}allon, {L}. and {L}inde, {D}. and {T}urbe-{D}oan, {A}. and {H}aon, {M}. and {D}aou, {M}. and {B}ertrand, {E}. and {F}aulds, {C}. {B}. and {S}ciara, {G}. and {A}damo, {M}. and {M}armeisse, {R}. and {C}omtet-{M}arre, {S}. and {P}eyret, {P}. and {A}brouk, {D}. and {R}uiz-{D}uenas, {F}. {J}. and {M}archand, {C}yril and {H}ugoni, {M}. and {L}uis, {P}. and {M}echichi, {T}. and {R}ecord, {E}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he functional diversity of the {N}ew {C}aledonian mangrove sediments was examined, observing the distribution of fungal dye-decolorizing peroxidases ({D}y{P}s), together with the complete biochemical characterization of the main {D}y{P}. {U}sing a functional metabarcoding approach, the diversity of expressed genes encoding fungal {D}y{P}s was investigated in surface and deeper sediments, collected beneath either {A}vicennia marina or {R}hizophora stylosa trees, during either the wet or the dry seasons. {T}he highest {D}y{P} diversity was observed in surface sediments beneath the {R}. stylosa area during the wet season, and one particular operational functional unit ({OFU}1) was detected as the most abundant {D}y{P} isoform. {T}his {OFU} was found in all sediment samples, representing 51-100% of the total {D}y{P}-encoding sequences in 70% of the samples. {T}he complete c{DNA} sequence corresponding to this abundant {D}y{P} ({OFU} 1) was retrieved by gene capture, cloned, and heterologously expressed in {P}ichia pastoris. {T}he recombinant enzyme, called {D}y{P}1, was purified and characterized, leading to the description of its physical-chemical properties, its ability to oxidize diverse phenolic substrates, and its potential to decolorize textile dyes; {D}y{P}1 was more active at low p{H}, though moderately stable over a wide p{H} range. {T}he enzyme was very stable at temperatures up to 50 degrees {C}, retaining 60% activity after 180 min incubation. {I}ts ability to decolorize industrial dyes was also tested on {R}eactive {B}lue 19, {A}cid {B}lack, {D}isperse {B}lue 79, and {R}eactive {B}lack 5. {T}he effect of hydrogen peroxide and sea salt on {D}y{P}1 activity was studied and compared to what is reported for previously characterized enzymes from terrestrial and marine-derived fungi.},
  keywords = {lignocellulose degrading enzymes ; dye-decolorizing peroxidases ; heterologous expression ; dye decolorization ; marine fungus ; mangrove ; salt adaptation ; {NOUVELLE} {CALEDONIE} ; {SAINT} {VINCENT} {BAIE}},
  booktitle = {},
  journal = {{J}ournal of {F}ungi},
  volume = {7},
  numero = {5},
  pages = {321 [23 ]},
  year = {2021},
  DOI = {10.3390/jof7050321},
  URL = {https://www.documentation.ird.fr/hor/fdi:010081517},
}