Quilbé J., Lamy Léo, Brottier Laurent, Leleux P., Fardoux Joël, Rivallan R., Benichou T., Guyonnet R., Becana M., Villar I., Garsmeur O., Hufnagel B., Delteil Amandine, Gully Djamel, Chaintreuil Clémence, Pervent M., Cartieaux Fabienne, Bourge M., Valentin N., Martin G., Fontaine L., Droc G., Dereeper Alexis, Farmer A., Libourel C., Nouwen Nico, Gressent F., Mournet P., D'Hont A., Giraud Eric, Klopp C., Arrighi Jean-François. (2021). Genetics of nodulation in Aeschynomene evenia uncovers mechanisms of the rhizobium-legume symbiosis. Nature Communications, 12 (1), 829 [14 p.]. ISSN 2041-1723.
Titre du document
Genetics of nodulation in Aeschynomene evenia uncovers mechanisms of the rhizobium-legume symbiosis
Année de publication
2021
Auteurs
Quilbé J., Lamy Léo, Brottier Laurent, Leleux P., Fardoux Joël, Rivallan R., Benichou T., Guyonnet R., Becana M., Villar I., Garsmeur O., Hufnagel B., Delteil Amandine, Gully Djamel, Chaintreuil Clémence, Pervent M., Cartieaux Fabienne, Bourge M., Valentin N., Martin G., Fontaine L., Droc G., Dereeper Alexis, Farmer A., Libourel C., Nouwen Nico, Gressent F., Mournet P., D'Hont A., Giraud Eric, Klopp C., Arrighi Jean-François
Source
Nature Communications, 2021,
12 (1), 829 [14 p.] ISSN 2041-1723
Among legumes (Fabaceae) capable of nitrogen-fixing nodulation, several Aeschynomene spp. use a unique symbiotic process that is independent of Nod factors and infection threads. They are also distinctive in developing root and stem nodules with photosynthetic bradyrhizobia. Despite the significance of these symbiotic features, their understanding remains limited. To overcome such limitations, we conduct genetic studies of nodulation in Aeschynomene evenia, supported by the development of a genome sequence for A. evenia and transcriptomic resources for 10 additional Aeschynomene spp. Comparative analysis of symbiotic genes substantiates singular mechanisms in the early and late nodulation steps. A forward genetic screen also shows that AeCRK, coding a receptor-like kinase, and the symbiotic signaling genes AePOLLUX, AeCCamK, AeCYCLOPS, AeNSP2, and AeNIN are required to trigger both root and stem nodulation. This work demonstrates the utility of the A. evenia model and provides a cornerstone to unravel mechanisms underlying the rhizobium-legume symbiosis. The establishment of symbiotic interaction between Aeschynomene evenia and photosynthetic bradyrhizobia doesn't involve the canonical Nod factors and infection threads. Here, the authors assemble the draft genome of A. evenia and identify a receptor-like kinase in mediating the symbiotic interaction.
Plan de classement
Biotechnologies [084]
Localisation
Fonds IRD [F B010080981]
Identifiant IRD
fdi:010080981
