@article{fdi:010080660, title = {{T}hreatened fish spawning area revealed by specific metabarcoding identification of eggs and larvae in the {B}eni {R}iver, upper {A}mazon}, author = {{M}iranda-{C}humacero, {G}. and {M}ariac, {C}{\'e}dric and {D}uponchelle, {F}abrice and {P}ainter, {L}. and {W}allace, {R}. and {C}ochonneau, {G}{\'e}rard and {M}olina-{R}odriguez, {J}. and {G}arcia-{D}avila, {C}. and {R}enno, {J}ean-{F}ran{\c{c}}ois}, editor = {}, language = {{ENG}}, abstract = {{I}dentifying fish spawning areas is of great ecological and conservation importance as fishes are suffering increasing threat levels from anthropogenic activities. {H}owever, to date very few studies have done so in the {A}mazon basin. {I}n the {B}eni {R}iver located in the upper {M}adeira basin, fishers reported that a particular ecotone near the town of {R}urrenabaque was a fish reproduction area. {T}o test the importance of this zone as a spawning site, we conducted an ichthyoplankton survey during the month when reproduction is most likely to occur. {T}he specific identification of larvae and eggs was made with a metabarcoding analysis. {W}ith this approach 13 different fish species of high importance for regional and local fisheries were identified, including the long-distance migratory gilded catfish ({B}rachyplatystoma rousseauxii), considered as endangered in the upper {M}adeira. {C}ombining the development time of morula-gastrula egg stages with the integrated current velocity of the river, we identified a spawning area that ranges between the last gravel beaches close to {A}ltamarani community and the {S}an {M}iguel del {B}ala community on top of {S}use strait, the last foothill of the {A}ndes. {T}his spawning area further extends upstream in the {M}adidi and {P}ilon {L}ajas protected areas and {T}acana and {T}'simane indigenous communities, when considering eggs in final embryo stage. {T}his portion of the {B}eni {R}iver, at the {A}ndean foothills, is heavily impacted by anthropogenic activities, ranging from unmanaged fisheries to contamination coming from the extraction of fluvial aggregates, upstream gold mining, deforestation associated with a sugar cane mill, an existing downstream dam, and projected upstream dam projects. {S}ome urgent solutions for the conservation of this already impacted area are proposed. {C}onfirming fish spawning zones, although difficult, is crucial to inform the definition of priority areas for conservation and management measures, in particular when these sites host endangered species.}, keywords = {{I}chthyoplankton ; {P}rochilodus ; {B}rachyplatystoma ; {Z}ungaro ; {D}ams ; {A}rid extraction ; {BOLIVIE} ; {AMAZONE} {BASSIN} ; {MADEIRA} {BASSIN} ; {BENI} {COURS} {D}'{EAU}}, booktitle = {}, journal = {{G}lobal {E}cology and {C}onservation}, volume = {24}, numero = {}, pages = {e01309 [11 ]}, ISSN = {2351-9894}, year = {2020}, DOI = {10.1016/j.gecco.2020.e01309}, URL = {https://www.documentation.ird.fr/hor/fdi:010080660}, }