Publications des scientifiques de l'IRD

Lavarenne J., Gonin M., Champion Antony, Javelle M., Adam Hélène, Rouster J., Conejero G., Lartaud M., Verdeil J. L., Laplaze Laurent, Sallaud C., Lucas Mikaël, Gantet Pascal. (2020). Transcriptome profiling of laser-captured crown root primordia reveals new pathways activated during early stages of crown root formation in rice. PLoS One, 15 (11), e0238736 [15 p.]. ISSN 1932-6203.

Titre du document
Transcriptome profiling of laser-captured crown root primordia reveals new pathways activated during early stages of crown root formation in rice
Année de publication
2020
Type de document
Article référencé dans le Web of Science WOS:000603518300004
Auteurs
Lavarenne J., Gonin M., Champion Antony, Javelle M., Adam Hélène, Rouster J., Conejero G., Lartaud M., Verdeil J. L., Laplaze Laurent, Sallaud C., Lucas Mikaël, Gantet Pascal
Source
PLoS One, 2020, 15 (11), e0238736 [15 p.] ISSN 1932-6203
Crown roots constitute the main part of the rice root system. Several key genes involved in crown root initiation and development have been identified by functional genomics approaches. Nevertheless, these approaches are impaired by functional redundancy and mutant lethality. To overcome these limitations, organ targeted transcriptome analysis can help to identify genes involved in crown root formation and early development. In this study, we generated an atlas of genes expressed in developing crown root primordia in comparison with adjacent stem cortical tissue at three different developmental stages before emergence, using laser capture microdissection. We identified 3975 genes differentially expressed in crown root primordia. About 30% of them were expressed at the three developmental stages, whereas 10.5%, 19.5% and 12.8% were specifically expressed at the early, intermediate and late stages, respectively. Sorting them by functional ontology highlighted an active transcriptional switch during the process of crown root primordia formation. Cross-analysis with other rice root development-related datasets revealed genes encoding transcription factors, chromatin remodeling factors, peptide growth factors, and cell wall remodeling enzymes that are likely to play a key role during crown root primordia formation. This atlas constitutes an open primary data resource for further studies on the regulation of crown root initiation and development.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020] ; Sciences du monde végétal [076]
Localisation
Fonds IRD [F B010080521]
Identifiant IRD
fdi:010080521
Contact