@article{fdi:010080444,
  title = {{T}ransferability, development of simple sequence repeat ({SSR}) markers and application to the analysis of genetic diversity and population structure of the {A}frican fan palm ({B}orassus aethiopum {M}art.) in {B}enin},
  author = {{K}patenon, {M}. {J}. and {S}alako, {K}. {V}. and {S}antoni, {S}. and {Z}ekraoui, {L}eila and {L}atreille, {M}. and {T}ollon-{C}ordet, {C}. and {M}ariac, {C}{\'e}dric and {J}aligot, {E}. and {B}eule, {T}. and {A}deoti, {K}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground {I}n {S}ub-{S}aharan {A}frica, {B}orassus aethiopum {M}art. ({A}frican fan palm) is an important non-timber forest product-providing palm that faces multiple anthropogenic threats to its genetic diversity. {H}owever, this species is so far under-studied, which prevents its sustainable development as a resource. {T}he present work is a first attempt at characterizing the genetic diversity and population structure of {B}. aethiopum across nine collection sites spanning the three climatic regions of {B}enin, {W}est {A}frica, through the use of microsatellite markers. {R}esults {D}uring a first phase we relied on the reported transferability of primers developed in other palm species. {W}e find that, in disagreement with previously published results, only 22.5% of the markers tested enable amplification of {B}. aethiopum {DNA} and polymorphism detection is very low. {I}n a second phase, we generated a {B}. aethiopum-specific genomic dataset through high-throughput sequencing and used it for the de novo detection of microsatellite loci. {A}mong the primer pairs targeting these, 11 detected polymorphisms and were further used for analyzing genetic diversity. {A}cross the nine sites, expected heterozygosity ({H}e) ranges from 0.263 to 0.451 with an overall average of 0.354, showing a low genetic diversity. {A}nalysis of molecular variance ({AMOVA}) shows that within-site variation accounts for 53% of the genetic variation. {A}ccordingly, the low number of migrants and positive values of the fixation index ({F}) in sites from both the {C}entral ({S}udano-{G}uinean) and the {S}outhern ({G}uinean) climatic regions suggest limited gene flow between sites. {T}he global correlation between genetic and geographic distances is weak; however, our clustering analyses indicate that {B}. aethiopum palms from {S}ave ({C}enter) are genetically more similar to those from the {N}orth than to samples from other {C}entral sites. {C}onclusions {I}n the light of our results, we discuss the use of inter-species transfer vs. de novo development of microsatellite markers in genetic diversity analyses targeting under-studied species, and suggest future applications for our molecular resources. {W}e propose that, while prominent short-range pollen and seed dispersal in {B}enin explain most of our results, gene flux between the {C}entral and {N}orthern regions, as a result of animal and/or human migrations, might underlie the {S}ave discrepancy.},
  keywords = {{B}orassus aethiopum ; {G}enetic diversity ; {M}icrosatellite ; {M}arker transferability ; {H}igh-throughput sequencing ; {S}imple sequence repeat ; {U}nder-studied species ; {BENIN}},
  booktitle = {},
  journal = {{BMC} {G}enetics},
  volume = {21},
  numero = {1},
  pages = {145 [23 ]},
  ISSN = {1471-2156},
  year = {2020},
  URL = {https://www.documentation.ird.fr/hor/fdi:010080444},
}