@article{fdi:010080018,
  title = {{M}olecular approach for the diagnosis of blood and skin canine filarioids},
  author = {{L}aidoudi, {Y}. and {B}edjaoui, {S}. and {M}edkour, {H}ac{\`e}ne and {L}atrofa, {M}. {S}. and {M}ekroud, {A}. and {B}itam, {I}. and {D}avoust, {B}. and {O}tranto, {D}. and {M}ediannikov, {O}leg},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he zoonotic {O}nchocerca lupi and tick-transmitted filarioids of the genus {C}ercopithifilaria remain less well known due to the difficulties in accessing to skin samples as target tissues. {H}ere, we proposed a molecular approach reliying on multiplex q{PCR} assays that allow the rapid identification of filarioids from canine blood, skin, and tick samples. {T}his includes two newly developed duplex q{PCR} tests, the first one targeting filarial and {C}. grassii {DNA} ({C}an{F}il-{C}. grassii). and the second q{PCR} assay designed for the detection of {C}ercopithifilaria bainae and {C}ercopithifilaria sp. {II} {DNA}s ({C}. bainae-{C}.sp{II}). {T}he third one is a triplex {T}aq{M}an cox 1 assay targeting {DNA} of blood microfilariae (e.g., {D}irofilaria immitis, {D}irofilaria repens and {A}canthocheilonema reconditum). {T}he novel duplex q{PCR}s developed were validated in silico and by screening of known {DNA} collection. {T}he q{PCR} assays were also used for screening the blood and tick samples of 72 dogs from {A}lgeria. {T}his allowed the identification of canine filariasis infection with 100% of specificity and 89.47% and 100% of sensitivity from naturally infected blood and tick samples, respectively. {T}he prevalences of 26.39% for {D}. immitis and 5.56% for both {D}. repens and {A}. reconditum were reported in blood and tick samples. {C}ercopithifilaria {DNA}s were detected only in tick samples, with a prevalence of 4.17% and 5.56% for {C}. bainae and {C}ercopithifilaria sp. {II}, respectively. {C}o-infections were diagnosed in 6.94% and 13.89% of blood and tick samples, respectively. {W}hereas all samples were negative for {C}. grassii {DNA}. {T}he use of engorged ticks instead of blood and skin samples could be an easier option for the surveillance of all canine filarioids herein investigated. {T}he multiplex q{PCR} assays herein validated were shown to be useful in the detection of filarial co-infections by overcoming sequencing of positive samples.},
  keywords = {{C}anine filarioses ; {D}irofilaria immitis ; {D}irofilaria repens ; {C}ercopithifilaria bainae ; {C}ercopithifilaria grassii ; {C}ercopithifilaria sp {II} ; {O}nchocerca lupi ; skin ; ticks ; multiplex q{PCR}},
  booktitle = {},
  journal = {{M}icroorganisms},
  volume = {8},
  numero = {11},
  pages = {1671 [15]},
  year = {2020},
  URL = {https://www.documentation.ird.fr/hor/fdi:010080018},
}