@article{fdi:010079886,
  title = {{S}pecies diversity and insecticide resistance within the {A}nopheles hyrcanus group in {U}bon {R}atchathani {P}rovince, {T}hailand},
  author = {{S}umarnrote, {A}. and {O}vergaard, {H}. {J}. and {C}orbel, {V}incent and {T}hanispong, {K}. and {C}hareonviriyaphap, {T}. and {M}anguin, {S}ylvie},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {M}embers of the{A}nopheles hyrcanusgroup have been incriminated as important malaria vectors. {T}his study aims to identify the species and explore the insecticide susceptibility profile within the {A}nopheles hyrcanus group in {U}bon {R}atchathani {P}rovince, northeastern {T}hailand where increasing numbers of malaria cases were reported in 2014. {M}ethods: {B}etween 2013 and 2015, five rounds of mosquito collections were conducted using human landing and cattle bait techniques during both the rainy and dry seasons. {A}nopheles mosquitoes were morphologically identified and their insecticide susceptibility status was investigated. {S}ynergist bioassays were carried out with {A}n. hyrcanus (s.l.) due to their resistance to all insecticides. {A}n {ITS}2-{PCR} assay was conducted to identify to species the {H}yrcanus group specimens. {R}esults: {O}ut of 10,361 {A}nopheles females collected, representing 18 taxa in 2 subgenera, 71.8% were morphologically identified as belonging to the {H}yrcanus {G}roup (subgenus {A}nopheles), followed by {A}n. barbirostris group (7.9%), {A}n. nivipes (6.5%), {A}n. philippinensis (5.9%) and the other 14 {A}nopheles species. {S}pecimens of the {H}yrcanus {G}roup were more prevalent during the rainy season and were found to be highly zoophilic. {A}nopheles hyrcanus (s.l.) was active throughout the night, with an early peak of activity between 18:00 h and 21:00 h. {ITS}2-{PCR} assay conducted on 603 {DNA} samples from specimens within the {H}yrcanus {G}roup showed the presence of five sisters species. {A}nopheles peditaeniatus was the most abundant species (90.5%,n= 546), followed by {A}n. nitidus (4.5%,n= 27), {A}n. nigerrimus (4.3%,n= 26), {A}n. argyropus (0.5%,n= 3), and {A}n. sinensis (0.2%,n= 1). {A}ll {A}n. hyrcanus (s.l.) specimens that were found resistant to insecticides (deltamethrin 0.05%, permethrin 0.75% and {DDT} 4% and synergist tests) belonged to {A}n. peditaeniatus. {T}he degree of resistance in {A}n. peditaeniatus to each of these three insecticides was approximately 50%. {A}ddition of {PBO} ({P}iperonyl butoxide), but not {DEF} ({S}.{S}.{S}-tributyl phosphotritioate), seemed to restore susceptibility, indicating a potential role of oxidases as a detoxifying enzyme resistance mechanism. {C}onclusions: {A} better understanding of mosquito diversity related to host preference, biting activity and insecticide resistance status will facilitate the implementation of locally adapted vector control strategies.},
  keywords = {{A}nopheles hyrcanus ; {M}alaria vectors ; {S}pecies diversity ; {I}nsecticide resistance ; {U}bon {R}atchathani {P}rovince ; {T}hailand ; {THAILANDE} ; {UBON} {RATCHATHANI} {PROVINCE}},
  booktitle = {},
  journal = {{P}arasites and {V}ectors},
  volume = {13},
  numero = {1},
  pages = {525 [13 p.]},
  ISSN = {1756-3305},
  year = {2020},
  DOI = {10.1186/s13071-020-04389-4},
  URL = {https://www.documentation.ird.fr/hor/fdi:010079886},
}