@article{fdi:010079742,
  title = {{G}enetic diversity of {P}lasmodium falciparum in {G}rande {C}omore {I}sland},
  author = {{M}ze, {N}. {P}. and {B}ogreau, {H}. and {D}iedhiou, {C}. {K}. and {H}erdell, {V}. and {R}ahamatou, {S}. and {B}ei, {A}. {K}. and {V}olkman, {S}. {K}. and {B}asco, {L}eonardo and {M}boup, {S}. and {A}houidi, {A}. {D}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground {D}espite several control interventions resulting in a considerable decrease in malaria prevalence in the {U}nion of the {C}omoros, the disease remains a public health problem with high transmission in {G}rande {C}omore compared to neighbouring islands. {I}n this country, only a few studies investigating the genetic diversity of {P}lasmodium falciparum have been performed so far. {F}or this reason, this study aims to examine the genetic diversity of {P}. falciparum by studying samples collected in {G}rande {C}omore in 2012 and 2013, using merozoite surface protein 1 (msp1), merozoite surface protein 2 (msp2) and single nucleotide polymorphism ({SNP}) genetic markers. {M}ethods {A} total of 162 positive rapid diagnostic test ({RDT}) samples from {G}rande {C}omore were used to extract parasite {DNA}. {A}llelic families {K}1, {M}ad20 and {RO}33 of the msp1 gene as well as allelic families {IC}3{D}7 and {FC}37 of the msp2 gene were determined by using nested {PCR}. {A}dditionally, 50 out of 151 samples were genotyped to study 24 {SNP}s by using high resolution melting ({HRM}).{R}esults{T}wo allelic families were predominant, the {K}1 family of msp1 gene (55%) and the {FC}27 family of msp2 gene (47.4%). {A}mong 50 samples genotyped for 24 {SNP}s, 42 (84%) yielded interpretable results. {O}ut of these isolates, 36 (85%) were genetically unique and 6 (15%) grouped into two clusters. {T}he genetic diversity of {P}. falciparum calculated from msp1 and msp2 genes and {SNP}s was 0.82 and 0.61, respectively. {C}onclusion {I}n summary, a large genetic diversity of {P}. falciparum was observed in {G}rande {C}omore. {T}his may favour persistence of malaria and might be one of the reasons for the high malaria transmission compared to neighbouring islands. {F}urther surveillance of {P}. falciparum isolates, mainly through environmental management and vector control, is warranted until complete elimination is attained.},
  keywords = {{M}alaria ; {SNP}s ; msp1 ; msp2 ; {C}omoros ; {COMORES}},
  booktitle = {},
  journal = {{M}alaria {J}ournal},
  volume = {19},
  numero = {1},
  pages = {320 [8p.]},
  year = {2020},
  DOI = {10.1186/s12936-020-03384-5},
  URL = {https://www.documentation.ird.fr/hor/fdi:010079742},
}