Benoist R., Capdevielle Dulac Claire, Chantre C., Jeannette Rémi, Calatayud Paul-André, Drezen J. M., Dupas Stéphane, Le Rouzic A., Le Rü Bruno, Moreau L., Van Dijk E., Kaiser L., Mougel F. (2020). Quantitative trait loci involved in the reproductive success of a parasitoid wasp. Molecular Ecology, 29 (18), 3476-3493. ISSN 0962-1083.
Titre du document
Quantitative trait loci involved in the reproductive success of a parasitoid wasp
Année de publication
2020
Auteurs
Benoist R., Capdevielle Dulac Claire, Chantre C., Jeannette Rémi, Calatayud Paul-André, Drezen J. M., Dupas Stéphane, Le Rouzic A., Le Rü Bruno, Moreau L., Van Dijk E., Kaiser L., Mougel F.
Source
Molecular Ecology, 2020,
29 (18), 3476-3493 ISSN 0962-1083
Dissecting the genetic basis of intraspecific variations in life history traits is essential to understand their evolution, notably for potential biocontrol agents. Such variations are observed in the endoparasitoid Cotesia typhae (Hymenoptera : Braconidae), specialized on the pest Sesamia nonagrioides (Lepidoptera : Noctuidae). Previously, we identified two strains of C. typhae that differed significantly for life history traits on an allopatric host population. To investigate the genetic basis underlying these phenotypic differences, we used a quantitative trait locus (QTL) approach based on restriction site-associated DNA markers. The characteristic of C. typhae reproduction allowed us generating sisters sharing almost the same genetic content, named clonal sibship. Crosses between individuals from the two strains were performed to generate F2 and F8 recombinant CSS. The genotypes of 181 clonal sibships were determined as well as the phenotypes of the corresponding 4,000 females. Informative markers were then used to build a high-quality genetic map. These 465 markers spanned a total length of 1,300 cM and were organized in 10 linkage groups which corresponded to the number of C. typhae chromosomes. Three QTLs were detected for parasitism success and two for offspring number, while none were identified for sex ratio. The QTLs explained, respectively, 27.7% and 24.5% of the phenotypic variation observed. The gene content of the genomic intervals was investigated based on the genome of C. congregata and revealed 67 interesting candidates, as potentially involved in the studied traits, including components of the venom and of the symbiotic virus (bracovirus) shown to be necessary for parasitism success in related wasps.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020]
;
Sciences du monde végétal [076]
;
Sciences du monde animal [080]
Localisation
Fonds IRD [F B010079527]
Identifiant IRD
fdi:010079527
