@article{fdi:010079456,
  title = {{I}dentification and structural characterization of the factors involved in vitellogenesis and its regulation in the {A}frican {O}steoglossiforme of aquacultural interest {H}eterotis niloticus ({C}uvier, 1829)},
  author = {{K}oua, {N}. {D}. and {N}unez {R}odriguez, {J}esus and {O}rjuela, {J}. and {Z}atylny-{G}audin, {C}. and {D}ubos, {M}. {P}. and {B}ernay, {B}. and {P}ontin, {J}. and {C}orre, {E}. and {H}enry, {J}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he {A}frican bonytongue ({H}eterotis niloticus) is an excellent candidate for fish farming because it has outstanding biological characteristics and zootechnical performances. {H}owever, the absence of sexual dimorphism does not favor its reproduction in captivity or the understanding of its reproductive behavior. {M}oreover, no molecular data related to its reproduction is yet available. {T}his study therefore focuses on the structural identification of the different molecular actors of vitellogenesis expressed in the pituitary gland, the liver and the ovary of {H}. niloticus. {A} transcriptomic approach based on de novo {RNA} sequencing of the pituitary gland, ovary and liver of females in vitellogenesis led to the creation of three transcriptomes. {I}n silico analysis of these transcriptomes identified the sequences of pituitary hormones such as prolactin ({PRL}), luteinizing hormone ({LH}) and follicle-stimulating hormone ({FSH}) and their ovarian receptors ({PRLR}, {FSHR}, {LHR}). {I}n the liver and ovary, estrogen receptors ({ER}) beta and gamma, liver vitellogenins ({V}tg{B} and {V}tg{C}) and their ovarian receptors ({VLDLR}) were identified. {F}inally, the partial transcript of an ovarian {V}tg weakly expressed compared to hepatic {V}tg was identified based on structural criteria. {M}oreover, a proteomic approach carried out from mucus revealed the presence of one {V}tg exclusively in females in vitellogenesis. {I}n this teleost fish that does not exhibit sexual dimorphism, mucus {V}tg could be used as a sexing biomarker based on a non-invasive technique compatible with the implementation of experimental protocols in vivo.},
  keywords = {{H}eterotis niloticus ; {R}eproduction ; {V}itellogenin ; {N}eurohormones ; {P}ituitary gland ; {E}stradiol receptors ; {T}ranscriptome ; {P}roteome},
  booktitle = {},
  journal = {{G}eneral and {C}omparative {E}ndocrinology},
  volume = {296},
  numero = {},
  pages = {113532 [30 ]},
  ISSN = {0016-6480},
  year = {2020},
  DOI = {10.1016/j.ygcen.2020.113532},
  URL = {https://www.documentation.ird.fr/hor/fdi:010079456},
}