@article{fdi:010079334,
  title = {{INT} toxicity over natural bacterial assemblages from surface oligotrophic waters : implications for the assessment of respiratory activity},
  author = {{B}anos, {I}. and {M}ontero, {M}. {F}. and {B}enavides, {M}ar and {A}ristegui, {J}.},
  editor = {},
  language = {{ENG}},
  abstract = {{P}lankton community respiration ({R}) is a major component of the carbon flux in aquatic ecosystems. {H}owever, current methods to measure actual respiration from oxygen consumption at relevant spatial scales are not sensitive enough in oligotrophic environments where respiration rates are very low. {T}o overcome this drawback, more sensitive indirect enzymatic approaches are commonly used as {R} proxies. {T}he in vivo electron transport system ({ETS}vivo) assay, which measures the reduction of (2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl tetrazolium chloride salt, {INT}) to {INT}-formazan in the presence of natural substrate levels, was recently proposed as an indirect reliable estimation of {R} for natural plankton communities. {H}owever, under in vivo conditions, formazan salts could be toxic to the cells. {H}ere, we test the toxicity of 0.2 m{M} of final {INT} concentration, widely used for {ETS}vivoassays, on natural bacterial assemblages collected in coastal and oceanic waters off {G}ran {C}anaria ({C}anary {I}slands, subtropical {N}orth {A}tlantic), in eight independent experiments. {A}fter 0.5 h of incubation, a significant but variable decline in cell viability (14-49%) was observed in all samples inoculated with {INT}. {M}oreover, {INT} also inhibited leucine uptake in less than 90 min of incubation. {I}n the light of these results, we argue that enzymatic respiratory rates obtained with the {ETS}vivomethod need to be interpreted with caution to derive {R} in oceanic regions where bacteria largely contribute to community respiration. {M}oreover, the variable toxicity on bacterial assemblages observed in our experiments questions the use of a single {R}/{ETS}vivorelationship as a universal proxy for regional studies.},
  keywords = {{ETS} ; {B}acterioplankton ; {C}ell viability ; {L}eucine incorporation ; {B}acterial respiration ; {ESPAGNE} ; {ATLANTIQUE} {NORD} ; {GRANDE} {CANARIE}},
  booktitle = {},
  journal = {{M}icrobial {E}cology},
  volume = {80},
  numero = {1},
  pages = {237--242},
  ISSN = {0095-3628},
  year = {2020},
  DOI = {10.1007/s00248-019-01479-4},
  URL = {https://www.documentation.ird.fr/hor/fdi:010079334},
}