@article{fdi:010079133,
  title = {{P}reparation of a sugarcane bagasse-based substrate for second-generation ethanol : effect of pasteurisation conditions on dephenolisation},
  author = {{G}onzalez-{B}autista, {E}. and {A}larcon-{G}utierrez, {E}. and {D}upuy, {N}. and {P}erraud {G}aime, {I}sabelle and {Z}iarelli, {F}. and {F}oli, {L}. and {F}arnet-{D}a-{S}ilva, {A}. {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{S}econd-generation ethanol process uses lignocellulose, but a pre-treatment is required to degrade lignin before saccharification. {B}iological pre-treatment, using phenoloxidases, represents a cheap and eco-friendly option. {M}ould contamination can be overcome by heat pre-conditioning of substrates. {P}asteurisation can be a mild and sustainable option. {A} 5-level response-surface experimental design was performed to test the effect of different time and temperatures on lignocellulolytic activities and substrate dephenolisation. {S}ubstrate microbial communities were characterised via catabolic profiles to determine functional diversity changes after pasteurisation. {T}emperatures ranging from 70 to 75 degrees {C} and time from 5 to 10 h led to the highest laccase activities and 30% of substrate dephenolisation was achieved while avoiding mould contamination. {T}he per cent of {O}-{A}lkyl (polysaccharides) did not vary significantly, meaning that polysaccharides were not extensively consumed. {M}icrobial communities were less catabolically diversified ({H}' = 1.2) but more active ({AWCD} = 0.9) and could degrade polysaccharides.},
  keywords = {{H}eat pre-treatment ; {L}ignocellulose transformation ; {S}olid-state fermentation ; {W}hite-rot fungi ; {L}accase},
  booktitle = {},
  journal = {{R}enewable {E}nergy},
  volume = {157},
  numero = {},
  pages = {859--866},
  ISSN = {0960-1481},
  year = {2020},
  DOI = {10.1016/j.renene.2020.05.116},
  URL = {https://www.documentation.ird.fr/hor/fdi:010079133},
}