@article{fdi:010078697,
  title = {{I}dentification and characterization of functional human monoclonal antibodies to {P}lasmodium vivax {D}uffy-binding protein},
  author = {{C}arias, {L}.{L}. and {D}echavanne, {S}. and {N}icolete, {C}.{V}. and {S}reng, {S}. and {S}uon, {S}. and {A}maratunga, {C}. and {F}airhurst, {R}.{M}. and {D}echavanne, {C}{\'e}lia and {B}arnes, {S}. and {W}itkowski, {B}. and {P}opovici, {J}. and {R}oesch, {C}. and {C}hen, {E}. and {F}erreira, {M}.{U}. and {T}olia, {N}.{H}. and {A}dams, {J}.{H}. and {K}ing, {C}.{L}.},
  editor = {},
  language = {{ENG}},
  abstract = {{P}lasmodium vivax invasion of reticulocytes relies on distinct receptor-ligand interactions between the parasite and host erythrocytes. {E}ngagement of the highly polymorphic domain {II} of the {P}. vivax {D}uffy-binding protein ({DBPII}) with the erythrocyte's {D}uffy {A}g receptor for chemokines ({DARC}) is essential. {S}ome {P} vivax-exposed individuals acquired {A}bs to {DBPII} that block {DBPIIDARC} interaction and inhibit {P} vivax reticulocyte invasion, and {A}b levels correlate with protection against {P}. vivax malaria. {T}o better understand the functional characteristics and fine specificity of protective human {A}bs to {DBPII}, we sorted single {DBPII}-specific {I}g{G}(+) memory {B} cells from three individuals with high blocking activity to {DBPII}. {W}e identified 12 {DBPII}-specific human m{A}bs from distinct lineages that blocked {DBPII}-{DARC} binding. {A}ll m{A}bs were {P}. vivax strain transcending and targeted known binding motifs of {DBPII} with {DARC}. {E}leven m{A}bs competed with each other for binding, indicating recognition of the same or overlapping epitopes. {N}aturally acquired blocking {A}bs to {DBPII} from individuals with high levels residing in different {P} vivax- endemic areas worldwide competed with m{A}bs, suggesting broadly shared recognition sites. {W}e also found that m{A}bs inhibited {P}. vivax entry into reticulocytes in vitro. {T}hese findings suggest that {I}g{G}(+) memory {B} cell activity in individuals with {P} vivax strain- transcending {A}bs to {DBPII} display a limited clonal response with inhibitory blocking directed against a distinct region of the molecule.},
  keywords = {},
  booktitle = {},
  journal = {{J}ournal of {I}mmunology},
  volume = {202},
  numero = {9},
  pages = {2648--2660},
  ISSN = {0022-1767},
  year = {2019},
  DOI = {10.4049/jimmunol.1801631},
  URL = {https://www.documentation.ird.fr/hor/fdi:010078697},
}