@article{fdi:010078593,
  title = {{D}etection and identification of trypanosomes by polymerase chain reaction in wild tsetse flies in {C}ameroon},
  author = {{M}orlais, {I}sabelle and {G}r{\'e}baut, {P}ascal and {B}odo, {J}.{M}. and {D}joba, {S}. and {C}uny, {G}{\'e}rard and {H}erder, {S}t{\'e}phane},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he prevalence of various species and subgroups of trypanosomes in infected flies from three sleeping sickness foci in {C}ameroon was determined by the use of polymerase chain reaction ({PCR}). {T}he predominant tsetse species found were {G}lossina palpalis palpalis. {M}icroscopical examination of 943 non-teneral tsetse flies revealed an average infection rate of 10.4%. {A} total of 90 flies were analyzed for trypanosome identification with primer sets specific for {T}rypanosoma ({T}rypanozoon) brucei s.l., {T}. ({D}uttonella) vivax, {T}. ({N}annomonas) simiae, and forest type {T}. ({N}annamonas) congolense. {PCR} succeeded in identifying 52 of the 90 infected flies. {O}ther primers were also tested on microscope positive/{PCR}-negative infections, and trypanosome subgroups were detected ({K}ilifi type and savannah type {T}. congolense). {PCR} amplification allowed identification of immature infections and revealed mixed-infections. {T}he {PCR} technique failed to identify 42.2% (38/90) of the parasitologically positive flies and the reasons for this failure are discussed.},
  keywords = {{TRYPANOSOMIASE} {HUMAINE} ; {MALADIE} {DU} {SOMMEIL} ; {VECTEUR} ; {ISOLEMENT} {D}'{AGENT} {PATHOGENE} ; {PREVALENCE} ; {PCR}.{REACTION} {DE} {POLYMERISATION} {EN} {CHAINE} ; {CAMEROUN}},
  booktitle = {},
  journal = {{A}cat {T}ropica},
  volume = {70},
  numero = {1},
  pages = {109--117},
  ISSN = {0001-706{X}},
  year = {1998},
  DOI = {10.1016/{S}0001-706{X}(98)00014-{X}},
  URL = {https://www.documentation.ird.fr/hor/fdi:010078593},
}