@article{fdi:010078209, title = {{S}urvey on non-human primates and mosquitoes does not provide evidences of spillover/spillback between the urban and sylvatic cycles of yellow fever and zika viruses following severe outbreaks in southeast {B}razil}, author = {de {A}breu, {F}. {V}. {S}. and {F}erreira-{D}e-{B}rito, {A}. and {A}zevedo, {A}. {D}. and {L}inhares, {J}. {H}. {R}. and {S}antos, {V}. {D}. and {M}iranda, {E}. {H}. and {N}eves, {M}sas and {Y}ousfi, {L}. and {R}ibeiro, {I}. {P}. and dos {S}antos, {A}. {A}. {C}. and dos {S}antos, {E}. and dos {S}antos, {T}. {P}. and {T}eixeira, {D}. {S}. and {G}omes, {M}. {Q}. and {F}ernandes, {C}. {B}. and da {S}ilva, {A}. {M}. {V}. and {L}ima, {M}. {D}. {Q}. and {P}aupy, {C}hristophe and {R}omano, {A}. {P}. {M}. and {B}om, {A}pda and de {O}liveira-{P}into, {L}. {M}. and {M}outailler, {S}. and {M}otta, {M}. {D}. and {C}astro, {M}. {G}. and {B}onaldo, {M}. {C}. and de {L}ima, {S}. {M}. {B}. and {L}ourenco-de-{O}liveira, {R}.}, editor = {}, language = {{ENG}}, abstract = {{I}n the last decade, {F}laviviruses such as yellow fever ({YFV}) and {Z}ika ({ZIKV}) have expanded their transmission areas. {T}hese viruses originated in {A}frica, where they exhibit both sylvatic and interhuman transmission cycles. {I}n {B}razil, the risk of {YFV} urbanization has grown, with the sylvatic transmission approaching the most densely populated metropolis, while concern about {ZIKV} spillback to a sylvatic cycle has risen. {T}o investigate these health threats, we carried out extensive collections and arbovirus screening of 144 free-living, non-human primates ({NHP}s) and 5219 mosquitoes before, during, and after {ZIKV} and {YFV} outbreaks (2015-2018) in southeast {B}razil. {ZIKV} infection was not detected in any {NHP} collected at any time. {I}n contrast, current and previous {YFV} infections were detected in {NHP}s sampled between 2017 and 2018, but not before the onset of the {YFV} outbreak. {M}osquito pools screened by high-throughput {PCR} were positive for {YFV} when captured in the wild and during the {YFV} outbreak, but were negative for 94 other arboviruses, including {ZIKV}, regardless of the time of collection. {I}n conclusion, there was no evidence of {YFV} transmission in coastal southeast {B}razil before the current outbreak, nor the spread or establishment of an independent sylvatic cycle of {ZIKV} or urban {A}edes aegypti transmission of {YFV} in the region. {I}n view of the region's receptivity and vulnerability to arbovirus transmission, surveillance of {NHP}s and mosquitoes should be strengthened and continuous.}, keywords = {arboviruses ; {F}lavivirus ; serology ; {PRNT} ; high throughput real time {PCR} ; {BRESIL}}, booktitle = {}, journal = {{V}iruses}, volume = {12}, numero = {4}, pages = {364 [21 ]}, year = {2020}, DOI = {10.3390/v12040364}, URL = {https://www.documentation.ird.fr/hor/fdi:010078209}, }