@article{fdi:010078004,
  title = {{T}argeted amplicon sequencing of 40 nuclear genes supports a single introduction and rapid radiation of {H}awaiian {M}etrosideros ({M}yrtaceae)},
  author = {{D}upuis, {J}. {R}. and {P}illon, {Y}ohan and {S}akishima, {T}. and {G}emmill, {C}. {E}. {C}. and {C}hamala, {S}. and {B}arbazuk, {W}. {B}. and {G}eib, {S}. {M}. and {S}tacy, {E}. {A}.},
  editor = {},
  language = {{ENG}},
  abstract = {{C}ompared to traditionally used plastid or ribosomal markers, nuclear gene markers provide many advantages for molecular systematics of plants, and high-throughput sequencing technologies are making large nuclear datasets available at an unprecedented rate. {W}e used targeted amplicon sequencing of 44 nuclear genes to construct a time-calibrated phylogeny of genus {M}etrosideros ({M}yrtaceae), evaluate recent systematic revisions, and assess whether phylogenetic signal within the {H}awaiian {A}rchipelago is correlated with island biogeography or morphological diversification. {W}e generated a final dataset of 40 nuclear genes for 187 specimens, used multiple search heuristics and species-tree analysis to estimate a phylogeny, and incorporated new fossils for the genus to estimate divergence times across the dataset. {A}ll analyses supported the monophyly of {M}etrosideros, including {C}arpolepis and {T}epualia. {H}awaiian {M}etrosideros were monophyletic and dated to 3.1 {MYA} using new fossils for the genus, which is intermediate to previous estimates based on nuclear ribosomal/chloroplast loci and calibrated with island ages. {W}ithin the {H}awaiian {M}etrosideros clade, we observed short branch lengths and unresolved relationships, and phylogenetic patterns were not concordant with biogeographic hypotheses of island progression, or the delineation of taxa or morphotypes. {A}verage nucleotide diversity was relatively consistent across the {H}awaiian {I}slands with the exception of slightly lower diversity on {K}aua'i. {T}hese results provide a data-rich estimate of the timing of a single introduction of {M}etrosideros to {H}awai'i and highlight the need for molecular markers with higher evolutionary rates for resolution of relationships within this recent radiation.},
  keywords = {{I}sland biogeography ; {M}olecular dating ; {N}ext-generation sequencing ; {P}acific {I}slands ; {P}hylogenomics ; {HAWAII} ; {NOUVELLE} {CALEDONIE} ; {NOUVELLE} {GUINEE} ; {NOUVELLE} {ZELANDE} ; {PHILIPPINES} ; {PACIFIQUE} {ILES} ; {SOLOMON} {CENTRAL} {ISLANDS}},
  booktitle = {},
  journal = {{P}lant {S}ystematics and {E}volution},
  volume = {305},
  numero = {10},
  pages = {961--974},
  ISSN = {0378-2697},
  year = {2019},
  DOI = {10.1007/s00606-019-01615-0},
  URL = {https://www.documentation.ird.fr/hor/fdi:010078004},
}