%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Toledo-Alarcon, J.
%A Cabrol, Léa
%A Jeison, D.
%A Trably, E.
%A Steyer, J. P.
%A Tapia-Venegas, E.
%T Impact of the microbial inoculum source on pre-treatment efficiency for fermentative H-2 production from glycerol
%D 2020
%L fdi:010077920
%G ENG
%J International Journal of Hydrogen Energy
%@ 0360-3199
%K Aeration treatment ; Biohydrogen ; Biokinetic control ; Dark fermentation ; Microbial community
%M ISI:000509629700022
%N 3
%P 1597-1607
%R 10.1016/j.ijhydene.2019.11.113
%U https://www.documentation.ird.fr/hor/fdi:010077920
%> https://www.documentation.ird.fr/intranet/publi/2020/03/010077920.pdf
%V 45
%W Horizon (IRD)
%X Hydrogen (H-2) production by dark fermentation can be performed from a wide variety of microbial inoculum sources, which are generally pre-treated to eliminate the activity of H-2 consuming species and/or enrich the microbial community with H-2-producing bacteria. This paper aims to study the impact of the microbial inoculum source on pre-treatment behavior, with a special focus on microbial community changes. Two inocula (aerobic and anaerobic sludge) and two pre-treatments (aeration and heat shock) were investigated using glycerol as substrate during a continuous operation. Our results show that the inoculum source significantly affected the pre-treatment efficiency. In aerobic sludge no pre-treatment is necessary, while in anaerobic sludge the heat pre-treatment increased H-2 production but aeration caused unstable H-2 production. In addition, biokinetic control was key in Clostridium selection as dominant species in all microbial communities. Lower and unstable H-2 production were associated with a higher relative abundance of Enterobacteriaceae family members. Our results allow a better understanding of H-2 production in continuous systems and how the microbial-community is affected. This provides key information for efficient selection of operating conditions for future applications.
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