@article{fdi:010077920,
  title = {{I}mpact of the microbial inoculum source on pre-treatment efficiency for fermentative {H}-2 production from glycerol},
  author = {{T}oledo-{A}larcon, {J}. and {C}abrol, {L}{\'e}a and {J}eison, {D}. and {T}rably, {E}. and {S}teyer, {J}. {P}. and {T}apia-{V}enegas, {E}.},
  editor = {},
  language = {{ENG}},
  abstract = {{H}ydrogen ({H}-2) production by dark fermentation can be performed from a wide variety of microbial inoculum sources, which are generally pre-treated to eliminate the activity of {H}-2 consuming species and/or enrich the microbial community with {H}-2-producing bacteria. {T}his paper aims to study the impact of the microbial inoculum source on pre-treatment behavior, with a special focus on microbial community changes. {T}wo inocula (aerobic and anaerobic sludge) and two pre-treatments (aeration and heat shock) were investigated using glycerol as substrate during a continuous operation. {O}ur results show that the inoculum source significantly affected the pre-treatment efficiency. {I}n aerobic sludge no pre-treatment is necessary, while in anaerobic sludge the heat pre-treatment increased {H}-2 production but aeration caused unstable {H}-2 production. {I}n addition, biokinetic control was key in {C}lostridium selection as dominant species in all microbial communities. {L}ower and unstable {H}-2 production were associated with a higher relative abundance of {E}nterobacteriaceae family members. {O}ur results allow a better understanding of {H}-2 production in continuous systems and how the microbial-community is affected. {T}his provides key information for efficient selection of operating conditions for future applications.},
  keywords = {{A}eration treatment ; {B}iohydrogen ; {B}iokinetic control ; {D}ark fermentation ; {M}icrobial community},
  booktitle = {},
  journal = {{I}nternational {J}ournal of {H}ydrogen {E}nergy},
  volume = {45},
  numero = {3},
  pages = {1597--1607},
  ISSN = {0360-3199},
  year = {2020},
  DOI = {10.1016/j.ijhydene.2019.11.113},
  URL = {https://www.documentation.ird.fr/hor/fdi:010077920},
}