Publications des scientifiques de l'IRD

Delrieu-Trottin E., Durand Jean-Dominique, Limmon G., Sukmono T., Kadarusman, Sugeha H. Y., Chen W. J., Busson Frédéric, Borsa Philippe, Dahruddin H., Sauri S., Fitriana Y., Zein M. S. A., Hocdé Régis, Pouyaud Laurent, Keith P., Wowor D., Steinke D., Hanner R., Hubert Nicolas. (2020). Biodiversity inventory of the grey mullets (Actinopterygii : Mugilidae) of the Indo-Australian Archipelago through the iterative use of DNA-based species delimitation and specimen assignment methods. Evolutionary Applications, 13 (6), 1451-1467. ISSN 1752-4571.

Titre du document
Biodiversity inventory of the grey mullets (Actinopterygii : Mugilidae) of the Indo-Australian Archipelago through the iterative use of DNA-based species delimitation and specimen assignment methods
Année de publication
2020
Type de document
Article référencé dans le Web of Science WOS:000512462700001
Auteurs
Delrieu-Trottin E., Durand Jean-Dominique, Limmon G., Sukmono T., Kadarusman, Sugeha H. Y., Chen W. J., Busson Frédéric, Borsa Philippe, Dahruddin H., Sauri S., Fitriana Y., Zein M. S. A., Hocdé Régis, Pouyaud Laurent, Keith P., Wowor D., Steinke D., Hanner R., Hubert Nicolas
Source
Evolutionary Applications, 2020, 13 (6), 1451-1467 ISSN 1752-4571
DNA barcoding opens new perspectives on the way we document biodiversity. Initially proposed to circumvent the limits of morphological characters to assign unknown individuals to known species, DNA barcoding has been used in a wide array of studies where collecting species identity constitutes a crucial step. The assignment of unknowns to knowns assumes that species are already well identified and delineated, making the assignment performed reliable. Here, we used DNA-based species delimitation and specimen assignment methods iteratively to tackle the inventory of the Indo-Australian Archipelago grey mullets, a notorious case of taxonomic complexity that requires DNA-based identification methods considering that traditional morphological identifications are usually not repeatable and sequence mislabeling is common in international sequence repositories. We first revisited a DNA barcode reference library available at the global scale for Mugilidae through different DNA-based species delimitation methods to produce a robust consensus scheme of species delineation. We then used this curated library to assign unknown specimens collected throughout the Indo-Australian Archipelago to known species. A second iteration of OTU delimitation and specimen assignment was then performed. We show the benefits of using species delimitation and specimen assignment methods iteratively to improve the accuracy of specimen identification and propose a workflow to do so.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020] ; Limnologie biologique / Océanographie biologique [034] ; Ecologie, systèmes aquatiques [036]
Description Géographique
PACIFIQUE
Localisation
Fonds IRD [F B010077906]
Identifiant IRD
fdi:010077906
Contact