Mai T., Toullec J., Van Wynsberge S., Besson M., Soulet S., Petek Sylvain, Aliotti E., Ekins M., Hall K., Erpenbeck D., Lecchini D., Beniddir M.A., Saulnier D., Debitus Cécile. (2019). Potential of fascaplysin and palauolide from Fascaplysinopsis cf reticulata to reduce the risk of bacterial infection in fish farming. Fisheries and Aquatic Sciences, 22, art. no 30 [11 p.]. ISSN 2234-1757.
Titre du document
Potential of fascaplysin and palauolide from Fascaplysinopsis cf reticulata to reduce the risk of bacterial infection in fish farming
Année de publication
2019
Auteurs
Mai T., Toullec J., Van Wynsberge S., Besson M., Soulet S., Petek Sylvain, Aliotti E., Ekins M., Hall K., Erpenbeck D., Lecchini D., Beniddir M.A., Saulnier D., Debitus Cécile
Source
Fisheries and Aquatic Sciences, 2019,
22, art. no 30 [11 p.] ISSN 2234-1757
Marine natural products isolated from the sponge Fascaplysinopsis cf reticulata, in French Polynesia, were investigated as an alternative to antibiotics to control pathogens in aquaculture. The overuse of antibiotics in aquaculture is largely considered to be an environmental pollution, because it supports the transfer of antibiotic resistance genes within the aquatic environment. One environmentally friendly alternative to antibiotics is the use of quorum sensing inhibitors (QSIs). Quorum sensing (QS) is a regulatory mechanism in bacteria which control virulence factors through the secretion of autoinducers (AIs), such as acyl-homoserine lactone (AHL) in gram-negative bacteria. Vibrio harveyi QS is controlled through three parallel pathways: HAI-1, AI-2, and CAI-1. Bioassay-guided purification of F. cf reticulata extract was conducted on two bacterial species, i.e., Tenacibaculum maritimum and V. harveyi for antibiotic and QS inhibition bioactivities. Toxicity bioassay of fractions was also evaluated on the freshwater fish Poecilia reticulata and the marine fish Acanthurus triostegus. Cyclohexanic and dichloromethane fractions of F. cf reticulata exhibited QS inhibition on V. harveyi and antibiotic bioactivities on V. harveyi and T. maritimum, respectively. Palauolide (1) and fascaplysin (2) were purified as major molecules from the cyclohexanic and dichloromethane fractions, respectively. Palauolide inhibited QS of V. harveyi through HAI-1 QS pathway at 50 µg ml-1 (26 µM), while fascaplysin affected the bacterial growth of V. harveyi (50 µg ml-1) and T. maritimum (0.25 µg). The toxicity of fascaplysin-enriched fraction (FEF) was evaluated and exhibited a toxic effect against fish at 50 µg ml-1. This study demonstrated for the first time the QSI potential of palauolide (1). Future research may assess the toxicity of both the cyclohexanic fraction of the sponge and palauolide (1) on fish, to confirm their potential as alternative to antibiotics in fish farming.
Plan de classement
Substances naturelles [035]
;
Ressources halieutiques [040]
Localisation
Fonds IRD [F B010077691]
Identifiant IRD
fdi:010077691
