@article{fdi:010077076,
  title = {{P}atterns of distribution, population genetics and ecological requirements of field-occurring resistant and susceptible {P}seudosuccinea columella snails to {F}asciola hepatica in {C}uba},
  author = {{A}lba, {A}. and {V}azquez, {A}. {A}. and {S}anchez, {J}. and {L}ounnas, {M}anon and {P}ointier, {J}. {P}. and {H}urtrez-{B}ousses, {S}. and {G}ourbal, {B}.},
  editor = {},
  language = {{ENG}},
  abstract = {{P}seudosuccinea columella snails transmit the trematode {F}asciola hepatica, but in {C}uba, six naturally occurring populations successfully resist parasite infection. {H}ere, we present an updated distribution of {P}. columella in {C}uba; 68 positive sites with the earliest records more abundant in west-central {C}uba and with east-central populations generally corresponding to the newest samples. {N}o records were found farther east. {T}he {IPA} site reported 10.5% prevalence of {F}. hepatica-infected snails. {P}opulation genetics, studied through microsatellites, showed low allelic and multilocus genotypic richness ({MLGT}), mainly in susceptible populations, strong deviations from panmixia and high self-fertilization rates. {S}usceptible individuals were grouped in one major cluster containing the majority of {MLGT}, and two independent clusters grouped the {MLGT} of resistant individuals from western and central populations, respectively. {F}rom these, we propose that several introductions of {P}. columella occurred in {C}uba, primarily in the west, with the early arrivals deriving on the resistant populations. {A} more recent introduction of susceptible {P}. columella carrying {MLGT} {T} and {Y} may have occurred, where the latter spread quickly through the island and possibly increase the risk of parasite transmission in {C}uba since all snails naturally infected with {F}. hepatica were carriers of the {MLGT} {Y}. {I}nterestingly, even though resistant populations are highly diverse and are likely the oldest within {C}uba, they are only found in six localities characterized by soft (total hardness, {TH} = 6.3 +/- 1.03 degrees d) and slightly acidic (p{H} = 6.2 +/- 0.12) waters with low richness in snail species (3.2 +/- 1.02). {T}his tendency was also observed in a two-year follow-up ecological study that was conducted on a farm where both phenotypes occurred in sympatry; colonization events by resistant over susceptible snails coincided with a reduction in the p{H} and {TH} of the water. {A} comparison of life traits in susceptible and resistant isolates reared at two different p{H}/{TH} conditions (5.9/4 degrees d or 7.8/14 degrees d) showed that low p{H}/{TH} negatively affects {P}. columella, irrespective of the phenotype. {H}owever, evidence of higher tolerance (higher survival, life expectancy, egg viability) to such conditions was observed in resistant isolates. {F}inally, we speculate that the limited distribution of resistant populations might be related to a better exploitation of sites that are less suitable to snails (thus, with lower competition), rather than to a differential ecological restriction to specific environmental conditions from susceptible {P}. columella.},
  keywords = {{CUBA}},
  booktitle = {},
  journal = {{S}cientific {R}eports - {N}ature},
  volume = {9},
  numero = {},
  pages = {art. 14359 [15 p.]},
  ISSN = {2045-2322},
  year = {2019},
  DOI = {10.1038/s41598-019-50894-7},
  URL = {https://www.documentation.ird.fr/hor/fdi:010077076},
}