@article{fdi:010076642,
  title = {{S}uspect and nontargeted strategies to investigate in vitro human biotransformation products of emerging environmental contaminants : the benzotriazoles},
  author = {{B}aduel, {C}hristine and {L}ai, {F}. {Y}. and van {N}uijs, {A}. {L}. {N}. and {C}ovaci, {A}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}enzotriazole derivatives ({BTR}s) are high production volume chemicals involved in a wide range of applications and consumer products resulting in their ubiquitous presence in environmental matrices. {Y}et, the human exposure assessment to these chemicals is limited since it is based only on the analysis of parent compounds in biological matrices. {T}he objective of this study was to investigate the in vitro human biotransformation for three widely used {BTR}s and to stepwise examine the role of {P}hase {I} and {II} enzymes (cytochrome {P}450 ({CYP}), uridine glucuronic acid transferase ({UGT}), and sulfotransferase ({SULT})) in their biotransformation. {E}xtracts with generated biotransformation products (bio{TP}s) were analyzed using liquid chromatography coupled to quadrupole-time-of-flight mass spectrometry ({LC}-{QTOF}-{MS}), followed by their identification based on a workflow combining suspect and nontargeted strategies. {T}en bio{TP}s were identified for 1{H}-benzotriazole, 14 for tolyltriazole, and 14 for 5-chloro-1-{H}-benzotriazole. {M}ost of the proposed bio{TP}s were identified and structurally elucidated for the first time. {B}ased on these findings, possible bio{TP}s and metabolic transformation pathways were subsequently predicted for other structurally close {BTR} derivatives. {O}ur findings provide new identified in vitro biotransformation products for future biomonitoring studies and emphasize that it is important to investigate the biotransformation pathway to assess overall exposure to xenobiotics.},
  keywords = {},
  booktitle = {},
  journal = {{E}nvironmental {S}cience and {T}echnology},
  volume = {53},
  numero = {17},
  pages = {10462--10469},
  ISSN = {0013-936{X}},
  year = {2019},
  DOI = {10.1021/acs.est.9b02429},
  URL = {https://www.documentation.ird.fr/hor/fdi:010076642},
}