@article{fdi:010076638,
  title = {{D}evelopment and evaluation of a duo {C}hikungunya virus {R}eal-{T}ime {RT}-{PCR} assay targeting two regions within the genome},
  author = {{T}hirion, {L}aurence and {P}ezzi, {L}. and {C}orcostegui, {I}. and {D}ubot {P}{\'e}r{\`e}s, {A}udrey and {F}alchi, {A}. and {L}amballerie, {X}. {D}e and {C}harrel, {R}. {N}.},
  editor = {},
  language = {{ENG}},
  abstract = {{C}hikungunya virus ({CHIKV}) re-emerged as a globalized health threat fifteen years ago. {T}here are dozens of {RT}-{PCR} assays published. {A}n inventory of the latter was made, and after in silico analysis, two assays were selected for their ability to detect strains belonging to the five {CHIKV} genetic lineages. {T}hey were combined in order to provide a robust assay not affected by genetic point mutations and the resulting {D}uo {CHIKV} real-time {RT}-{PCR} assay was compared to the two parental single-plex tests against five strains belonging to the five genetic lineages. {T}he {D}uo {CHIKV} assay performed equally, or better, in terms of sensitivity, specificity, linearity and signal intensity. {D}ual-target assays are better suited for viruses having the propensity to evolve into new variants via point mutations or major sequence deletions/insertions. {H}ere, we demonstrated that combining two single systems into a dual-target assay did not impair sensitivity and specificity, and proved a potent diagnostic tool to face a potential emergence of {CHIKV} variants by newly evolving mutations.},
  keywords = {{A}lphavirus ; {T}ogaviridae ; emergence ; arbovirus ; arthropod-borne ; fever ; epidemic ; outbreak ; tropical disease ; {AFRIQUE} ; {ASIE}},
  booktitle = {},
  journal = {{V}iruses},
  volume = {11},
  numero = {8},
  pages = {art. 755 [12 ]},
  year = {2019},
  DOI = {10.3390/v11080755},
  URL = {https://www.documentation.ird.fr/hor/fdi:010076638},
}