Trinh D. C., Lavenus J., Goh T., Boutte Y., Drogue Q., Vaissayre Virginie, Tellier F., Lucas Mikaël, Voss U., Gantet Pascal, Faure J. D., Dussert Stéphane, Fukaki H., Bennet M. J., Laplaze Laurent, Guyomarc'h S. (2019). PUCHI regulates very long chain fatty acid biosynthesis during lateral root and callus formation. Proceedings of the National Academy of Sciences of the United States of America, 116 (28), p. 14325-14330. ISSN 0027-8424.
Titre du document
PUCHI regulates very long chain fatty acid biosynthesis during lateral root and callus formation
Année de publication
2019
Auteurs
Trinh D. C., Lavenus J., Goh T., Boutte Y., Drogue Q., Vaissayre Virginie, Tellier F., Lucas Mikaël, Voss U., Gantet Pascal, Faure J. D., Dussert Stéphane, Fukaki H., Bennet M. J., Laplaze Laurent, Guyomarc'h S.
Source
Proceedings of the National Academy of Sciences of the United States of America, 2019,
116 (28), p. 14325-14330 ISSN 0027-8424
Lateral root organogenesis plays an essential role in elaborating plant root system architecture. In Arabidopsis, the AP2 family transcription factor PUCHI controls cell proliferation in lateral root primordia. To identify potential targets of PUCHI, we analyzed a time course transcriptomic dataset of lateral root formation. We report that multiple genes coding for very long chain fatty acid (VLCFA) biosynthesis enzymes are induced during lateral root development in a PUCHI-dependent manner. Significantly, several mutants perturbed in VLCFA biosynthesis show similar lateral root developmental defects as puchi-1. Moreover, puchi-1 roots display the same disorganized callus formation phenotype as VLCFA biosynthesis-deficient mutants when grown on auxin-rich callus-inducing medium. Lipidomic profiling of puchi-1 roots revealed reduced VLCFA content compared with WT. We conclude that PUCHI-regulated VLCFA biosynthesis is part of a pathway controlling cell proliferation during lateral root and callus formation.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020]
;
Sciences du monde végétal [076]
Localisation
Fonds IRD [F B010076216]
Identifiant IRD
fdi:010076216
