@article{fdi:010075716,
  title = {{A} multiplex {PCR} assay for the identification of five species of the {A}nopheles barbirostris complex in {T}hailand},
  author = {{B}rosseau, {L}. and {U}dom, {C}. and {S}ukkanon, {C}. and {C}hareonviriyaphap, {T}. and {B}angs, {M}. {J}. and {S}aeung, {A}. and {M}anguin, {S}ylvie},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground{T}he {B}arbirostris {C}omplex comprises six formally described species that cannot be differentiated based on morphology alone. {O}ut of these six species, two have been reported as putative malaria vectors, {A}n. campestris and {A}n. wejchoochotei. {F}ive species are present in {T}hailand, {A}n. barbirostris, {A}n. campestris, {A}n. dissidens, {A}n. saeungae and {A}n. wejchoochotei, while {A}n. vanderwulpi occurs in {I}ndonesia. {A}s these species cannot be accurately differentiated by morphological characters, there is a crucial lack of information on their bionomics and role in the transmission of malaria and filariasis agents.{R}esults{F}or differentiating the six species, an allele-specific amplification ({AS}-{PCR}) based on the second internal transcribed spacer ({ITS}2) sequence was developed. {F}rom 862 mosquitoes in the {B}arbirostris {C}omplex collected in 23 provinces throughout {T}hailand, the {AS}-{PCR} was able to identify five species and its validation was undertaken on 185 specimens.{C}onclusions{T}his multiplex-{PCR} assay is potentially able to definitely identify all six species of the {B}arbirostris {C}omplex and was validated on five species present in {T}hailand.},
  keywords = {{A}nopheles ; {B}arbirostris {C}omplex ; {ITS}2 ; multiplex {PCR} ; {T}hailand ; {THAILANDE}},
  booktitle = {},
  journal = {{P}arasites and {V}ectors},
  volume = {12},
  numero = {},
  pages = {art. 223 [10 p.]},
  ISSN = {1756-3305},
  year = {2019},
  DOI = {10.1186/s13071-019-3494-8},
  URL = {https://www.documentation.ird.fr/hor/fdi:010075716},
}