@article{fdi:010075713,
  title = {{L}ong-fragment targeted capture for long-read sequencing of plastomes},
  author = {{B}ethune, {K}{\'e}vin and {M}ariac, {C}{\'e}dric and {C}ouderc, {M}arie and {S}carcelli, {N}ora and {S}antoni, {S}. and {A}rdisson, {M}. and {M}artin, {J}. {F}. and {M}ontufar, {R}. and {K}lein, {V}. and {S}abot, {F}ran{\c{c}}ois and {V}igouroux, {Y}ves and {C}ouvreur, {T}homas},
  editor = {},
  language = {{ENG}},
  abstract = {{P}remise {T}hird-generation sequencing methods generate significantly longer reads than those produced using alternative sequencing methods. {T}his provides increased possibilities for the study of biodiversity, phylogeography, and population genetics. {W}e developed a protocol for in-solution enrichment hybridization capture of long {DNA} fragments applicable to complete plastid genomes. {M}ethods and {R}esults {T}he protocol uses cost-effective in-house probes developed via long-range {PCR} and was used in six non-model monocot species ({P}oaceae: {A}frican rice, pearl millet, fonio; and three palm species). {DNA} was extracted from fresh and silica gel-dried leaves. {O}ur protocol successfully captured long-read plastome fragments (3151 bp median on average), with an enrichment rate ranging from 15% to 98%. {DNA} extracted from silica gel-dried leaves led to low-quality plastome assemblies when compared to {DNA} extracted from fresh tissue. {C}onclusions {O}ur protocol could also be generalized to capture long sequences from specific nuclear fragments.},
  keywords = {de novo assembly ; {DNA} probes ; long-range {PCR} ; {M}in{ION} ; whole plastome ; sequencing},
  booktitle = {},
  journal = {{A}pplications in {P}lant {S}ciences},
  volume = {7},
  numero = {5},
  pages = {e1243 [13 ]},
  ISSN = {2168-0450},
  year = {2019},
  DOI = {10.1002/aps3.1243},
  URL = {https://www.documentation.ird.fr/hor/fdi:010075713},
}