@article{fdi:010075685, title = {{T}owards a global {DNA} barcode reference library for quarantine identifications of lepidopteran stemborers, with an emphasis on sugarcane pests}, author = {{L}ee, {T}. {R}. {C}. and {A}nderson, {S}. {J}. and {T}ran-{N}guyen, {L}. {T}. {T}. and {S}allam, {N}. and {L}e {R}ΓΌ, {B}runo and {C}onlong, {D}. and {P}owell, {K}. and {W}ard, {A}. and {M}itchell, {A}.}, editor = {}, language = {{ENG}}, abstract = {{L}epidopteran stemborers are among the most damaging agricultural pests worldwide, able to reduce crop yields by up to 40%. {S}ugarcane is the world's most prolific crop, and several stemborer species from the families {N}octuidae, {T}ortricidae, {C}rambidae and {P}yralidae attack sugarcane. {A}ustralia is currently free of the most damaging stemborers, but biosecurity efforts are hampered by the difficulty in morphologically distinguishing stemborer species. {H}ere we assess the utility of {DNA} barcoding in identifying stemborer pest species. {W}e review the current state of the {COI} barcode sequence library for sugarcane stemborers, assembling a dataset of 1297 sequences from 64 species. {S}equences were from specimens collected and identified in this study, downloaded from {BOLD} or requested from other authors. {W}e performed species delimitation analyses to assess species diversity and the effectiveness of barcoding in this group. {S}even species exhibited < 0.03 {K}2{P} interspecific diversity, indicating that diagnostic barcoding will work well in most of the studied taxa. {W}e identified 24 instances of identification errors in the online database, which has hampered unambiguous stemborer identification using barcodes. {I}nstances of very high within-species diversity indicate that nuclear markers (e.g. 18{S}, 28{S}) and additional morphological data (genitalia dissection of all lineages) are needed to confirm species boundaries.}, keywords = {}, booktitle = {}, journal = {{S}cientific {R}eports - {N}ature}, volume = {9}, numero = {}, pages = {art. 7039 [19 p.]}, ISSN = {2045-2322}, year = {2019}, DOI = {10.1038/s41598-019-42995-0}, URL = {https://www.documentation.ird.fr/hor/fdi:010075685}, }