@article{fdi:010075641,
  title = {{V}iral {RNA} degradation makes urine a challenging specimen for detection of {J}apanese {E}ncephalitis {V}irus in patients with suspected {CNS} infection},
  author = {{B}harucha, {T}. and {S}engvilaipaseuth, {O}. and {S}eephonelee, {M}. and {V}ongsouvath, {M}. and {V}ongsouvath, {M}. and {R}attanavong, {S}. and {P}iorkowski, {G}. and {L}ecuit, {M}. and {G}orman, {C}. and {P}ommier, {J}. {D}. and {G}arson, {J}. {A}. and {N}ewton, {P}. {N}. and de {L}amballerie, {X}. and {D}ubot {P}{\'e}r{\`e}s, {A}udrey},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground. {J}apanese encephalitis virus ({JEV}) is a leading cause of central nervous system ({CNS}) infections in {A}sia and results in significant morbidity and mortality. {JEV} {RNA} is rarely detected in serum or cerebrospinal fluid ({CSF}), and diagnosis of {JEV} infection is usually based on serological tests that are frequently difficult to interpret. {U}nlike serum or {CSF}, urine is relatively easy to obtain, but, to date, there has been minimal work on the feasibility of testing urine for {JEV} {RNA}. {M}ethods. {W}e investigated the use of lysis buffer and a {M}icrosep device to optimize urine storage for detection of {JEV} {RNA} by reverse transcription real-time polymerase chain reaction ({RT}-q{PCR}). {T}he best of the studied methods was then evaluated in consecutive patients admitted to the hospital with suspected {CNS} infections in {L}aos. {R}esults. {W}e demonstrated degradation of {JEV} {RNA} in urine after even short storage periods at 4 degrees {C} or-80 degrees {C}. {A}lthough there was no advantage in using a {M}icrosep concentration device alone, immediate addition of lysis buffer to fresh urine improved the detection of {JEV} {RNA} at the limit of detection. {C}onclusions. {I}n 2 studies of 41 patients with acute encephalitis syndrome, 11 (27%) were positive for {JEV} {I}g{M} in {CSF} and/or serum, and 2 (4.9%) were {JEV} {RT}-q{PCR} positive from throat swabs. {JEV} {RNA} was not detected in any of these patients' urine samples. {H}owever, lysis buffer was only used during a prospective study, that is, for only 17/41 (41%) patient urine samples. {O}ur findings suggest a need for larger studies testing urine for {JEV} {RNA}, with urine collected at different times from symptom onset, and using lysis buffer, which stabilizes {RNA}, for storage.},
  keywords = {diagnosis ; flavivirus ; {J}apanese encephalitis virus ; {RNA} ; {RT}-q{PCR} ; {LAOS}},
  booktitle = {},
  journal = {{O}pen {F}orum {I}nfectious {D}iseases},
  volume = {6},
  numero = {3},
  pages = {ofz048 [8 p.]},
  ISSN = {2328-8957},
  year = {2019},
  DOI = {10.1093/ofid/ofz048},
  URL = {https://www.documentation.ird.fr/hor/fdi:010075641},
}