Horizon / Plein textes La base de ressources documentaires de l'IRD

IRD

 

Publications des scientifiques de l'IRD

Bharucha T., Sengvilaipaseuth O., Seephonelee M., Vongsouvath M., Vongsouvath M., Rattanavong S., Piorkowski G., Lecuit M., Gorman C., Pommier J. D., Garson J. A., Newton P. N., de Lamballerie X., Dubot Pérès Audrey. (2019). Viral RNA degradation makes urine a challenging specimen for detection of Japanese Encephalitis Virus in patients with suspected CNS infection. Open Forum Infectious Diseases, 6 (3), ofz048 [8 p.]. ISSN 2328-8957

Fichier PDF disponiblehttp://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers19-05/010075641.pdf[ PDF Link ]

Lien direct chez l'éditeur doi:10.1093/ofid/ofz048

Titre
Viral RNA degradation makes urine a challenging specimen for detection of Japanese Encephalitis Virus in patients with suspected CNS infection
Année de publication2019
Type de documentArticle référencé dans le Web of Science WOS:000465327200020
AuteursBharucha T., Sengvilaipaseuth O., Seephonelee M., Vongsouvath M., Vongsouvath M., Rattanavong S., Piorkowski G., Lecuit M., Gorman C., Pommier J. D., Garson J. A., Newton P. N., de Lamballerie X., Dubot Pérès Audrey.
SourceOpen Forum Infectious Diseases, 2019, 6 (3), p. ofz048 [8 p.]. p. ofz048 [8 p.] ISSN 2328-8957
RésuméBackground. Japanese encephalitis virus (JEV) is a leading cause of central nervous system (CNS) infections in Asia and results in significant morbidity and mortality. JEV RNA is rarely detected in serum or cerebrospinal fluid (CSF), and diagnosis of JEV infection is usually based on serological tests that are frequently difficult to interpret. Unlike serum or CSF, urine is relatively easy to obtain, but, to date, there has been minimal work on the feasibility of testing urine for JEV RNA. Methods. We investigated the use of lysis buffer and a Microsep device to optimize urine storage for detection of JEV RNA by reverse transcription real-time polymerase chain reaction (RT-qPCR). The best of the studied methods was then evaluated in consecutive patients admitted to the hospital with suspected CNS infections in Laos. Results. We demonstrated degradation of JEV RNA in urine after even short storage periods at 4 degrees C or-80 degrees C. Although there was no advantage in using a Microsep concentration device alone, immediate addition of lysis buffer to fresh urine improved the detection of JEV RNA at the limit of detection. Conclusions. In 2 studies of 41 patients with acute encephalitis syndrome, 11 (27%) were positive for JEV IgM in CSF and/or serum, and 2 (4.9%) were JEV RT-qPCR positive from throat swabs. JEV RNA was not detected in any of these patients' urine samples. However, lysis buffer was only used during a prospective study, that is, for only 17/41 (41%) patient urine samples. Our findings suggest a need for larger studies testing urine for JEV RNA, with urine collected at different times from symptom onset, and using lysis buffer, which stabilizes RNA, for storage.
Plan de classementEntomologie médicale / Parasitologie / Virologie [052]
Descr. géo.LAOS
LocalisationFonds IRD [F B010075641]
Identifiant IRDfdi:010075641
Lien permanenthttp://www.documentation.ird.fr/hor/fdi:010075641

Export des données

Disponibilité des documents

Télechargment fichier PDF téléchargeable

Lien sur le Web lien chez l'éditeur

Accès réservé en accès réservé

HAL en libre accès sur HAL


* PDF Link :

    à télécharger pour citer/partager ce document sur les réseaux sociaux académiques


Accès aux documents originaux :

Le FDI est labellisé CollEx

Accès direct

Bureau du chercheur

Site de la documentation

Espace intranet IST (accès réservé)

Suivi des publications IRD (accès réservé)

Mentions légales

Services Horizon

Poser une question

Consulter l'aide en ligne

Déposer une publication (accès réservé)

S'abonner au flux RSS

Voir les tableaux chronologiques et thématiques

Centres de documentation

Bondy

Montpellier (centre IRD)

Montpellier (MSE)

Cayenne

Nouméa

Papeete

Abidjan

Dakar

Niamey

Ouagadougou

Tunis

La Paz

Quito