@article{fdi:010075600,
  title = {{E}nvironmental variations in {M}ycobacterium ulcerans transcriptome : absence of mycolactone expression in suboptimal environments},
  author = {{S}anhueza, {D}aniel and {G}u{\'e}gan, {J}ean-{F}ran{\c{c}}ois and {J}ordan, {H}. and {C}hevillon, {C}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}uruli ulcer is a neglected tropical infectious disease, produced by the environmentally persistent pathogen {M}ycobacterium ulcerans ({MU}). {N}either the ecological niche nor the exact mode of transmission of {MU} are completely elucidated. {H}owever, some environmental factors, such as the concentration in chitin and p{H} values, were reported to promote {MU} growth in vitro. {W}e pursued this research using next generation sequencing ({NGS}) and m{RNA} sequencing to investigate potential changes in {MU} genomic expression profiles across in vitro environmental conditions known to be suitable for {MU} growth. {S}upplementing the growth culture medium in either chitin alone, calcium alone, or in both chitin and calcium significantly impacted the {MU} transcriptome and thus several metabolic pathways, such as, for instance, those involved in {DNA} synthesis or cell wall production. {B}y contrast, some genes carried by the virulence plasmid and necessary for the production of the mycolactone toxin were expressed neither in control nor in any modified environments. {W}e hypothesized that these genes are only expressed in stressful conditions. {O}ur results describe important environmental determinants playing a role in the pathogenicity of {MU}, helping the understanding of its complex natural life cycle and encouraging further research using genomic approaches.},
  keywords = {{M}ycobacterium ulcerans ; {B}uruli ulcer ; infectious disease ; ecological niche ; ecological transmission ; m{RNA}-sequencing},
  booktitle = {},
  journal = {{T}oxins},
  volume = {11},
  numero = {3},
  pages = {art. 146 [14 p.]},
  ISSN = {2072-6651},
  year = {2019},
  DOI = {10.3390/toxins11030146},
  URL = {https://www.documentation.ird.fr/hor/fdi:010075600},
}