@article{fdi:010075575,
  title = {{U}ltraspecific live imaging of the dynamics of zebrafish neutrophil granules by a histopermeable fluorogenic benzochalcone probe},
  author = {{C}olucci-{G}uyon, {E}. and {B}atista, {A}. {S}. and {O}liveira, {S}. {D}. {S}. and {B}laud, {M}. and {B}ellettini, {I}. {C}. and {M}arteyn, {B}. {S}. and {L}eblanc, {K}. and {H}erbomel, {P}. and {D}uval, {R}omain},
  editor = {},
  language = {{ENG}},
  abstract = {{N}eutrophil granules ({NG}s) are key components of the innate immune response and mark the development of neutrophilic granulocytes in mammals. {H}owever, there has been no specific fluorescent vital stain up to now to monitor their dynamics within a whole live organism. {W}e rationally designed a benzochalcone fluorescent probe ({HAB}) featuring high tissue permeability and optimal photophysics such as elevated quantum yield, pronounced solvatochromism and target-induced fluorogenesis. {P}henotypic screening identified {HAB} as the first cell- and organelle-specific small-molecule fluorescent tracer of {NG}s in live zebrafish larvae, with no labeling of other cell types or organelles. {HAB} staining was independent of the state of neutrophil activation, labeling {NG}s of both resting and phagocytically active neutrophils with equal specificity. {B}y high-resolution live imaging, we documented the dynamics of {HAB}-stained {NG}s during phagocytosis. {U}pon zymosan injection, labeled {NG}s were rapidly recruited to the forming phagosomes. {D}espite being a reversible ligand, {HAB} could not be displaced by high concentrations of pharmacologically relevant competing chalcones, indicating that this specific labeling was the result of the {HAB}'s precise physicochemical signature rather than a general feature of chalcones. {H}owever, one of the competitors was discovered as a promising interstitial fluorescent tracer illuminating zebrafish histology, similarly to {BODIPY}-ceramide. {A}s a yellow-emitting histopermeable vital stain, {HAB} functionally and spectrally complements most genetically incorporated fluorescent tags commonly used in live zebrafish biology, holding promise for the study of neutrophil-dependent responses relevant to human physiopathology such as developmental defects, inflammation and infection. {F}urthermore, {HAB} intensely labeled isolated live human neutrophils at the level of granulated subcellular structures consistent with human {NG}s, suggesting that the labeling of {NG}s by {HAB} is not restricted to the zebrafish model but also relevant to mammalian systems.},
  keywords = {},
  booktitle = {},
  journal = {{C}hemical {S}cience},
  volume = {10},
  numero = {12},
  pages = {3654--3670},
  ISSN = {2041-6520},
  year = {2019},
  DOI = {10.1039/c8sc05593a},
  URL = {https://www.documentation.ird.fr/hor/fdi:010075575},
}