Horizon / Plein textes La base de ressources documentaires de l'IRD

IRD

 

Publications des scientifiques de l'IRD

Dietrich Muriel, Markotter W. (2019). Studying the microbiota of bats : accuracy of direct and indirect samplings. Ecology and Evolution, 9 (4), 1730-1735. ISSN 2045-7758

Fichier PDF disponiblehttp://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers19-04/010075465.pdf[ PDF Link ]

Lien direct chez l'éditeur doi:10.1002/ece3.4842

Titre
Studying the microbiota of bats : accuracy of direct and indirect samplings
Année de publication2019
Type de documentArticle référencé dans le Web of Science WOS:000461114900016
AuteursDietrich Muriel, Markotter W.
SourceEcology and Evolution, 2019, 9 (4), p. 1730-1735. ISSN 2045-7758
RésuméGiven the recurrent bat-associated disease outbreaks in humans and recent advances in metagenomics sequencing, the microbiota of bats is increasingly being studied. However, obtaining biological samples directly from wild individuals may represent a challenge, and thus, indirect passive sampling (without capturing bats) is sometimes used as an alternative. Currently, it is not known whether the bacterial community assessed using this approach provides an accurate representation of the bat microbiota. This study was designed to compare the use of direct sampling (based on bat capture and handling) and indirect sampling (collection of bat's excretions under bat colonies) in assessing bacterial communities in bats. Using high-throughput 16S rRNA sequencing of urine and feces samples from Rousettus aegyptiacus, a cave-dwelling fruit bat species, we found evidence of niche specialization among different excreta samples, independent of the sampling approach. However, sampling approach influenced both the alpha- and beta-diversity of urinary and fecal microbiotas. In particular, increased alpha-diversity and more overlapping composition between urine and feces samples was seen when direct sampling was used, suggesting that cross-contamination may occur when collecting samples directly from bats in hand. In contrast, results from indirect sampling in the cave may be biased by environmental contamination. Our methodological comparison suggested some influence of the sampling approach on the bat-associated microbiota, but both approaches were able to capture differences among excreta samples. Assessment of these techniques opens an avenue to use more indirect sampling, in order to explore microbial community dynamics in bats.
Plan de classementSciences du monde animal [080] ; Biotechnologies [084]
Descr. géo.AFRIQUE DU SUD
LocalisationFonds IRD [F B010075465]
Identifiant IRDfdi:010075465
Lien permanenthttp://www.documentation.ird.fr/hor/fdi:010075465

Export des données

Disponibilité des documents

Télechargment fichier PDF téléchargeable

Lien sur le Web lien chez l'éditeur

Accès réservé en accès réservé

HAL en libre accès sur HAL


* PDF Link :

    à télécharger pour citer/partager ce document sur les réseaux sociaux académiques


Accès aux documents originaux :

Le FDI est labellisé CollEx

Accès direct

Bureau du chercheur

Site de la documentation

Espace intranet IST (accès réservé)

Suivi des publications IRD (accès réservé)

Mentions légales

Services Horizon

Poser une question

Consulter l'aide en ligne

Déposer une publication (accès réservé)

S'abonner au flux RSS

Voir les tableaux chronologiques et thématiques

Centres de documentation

Bondy

Montpellier (centre IRD)

Montpellier (MSE)

Cayenne

Nouméa

Papeete

Abidjan

Dakar

Niamey

Ouagadougou

Tunis

La Paz

Quito