@article{fdi:010075180,
  title = {{N}ew perspectives on nitrogen fixation measurements using {N}-15(2) gas},
  author = {{W}annicke, {N}. and {B}enavides, {M}ar and {D}alsgaard, {T}. and {D}ippner, {J}. {W}. and {M}ontoya, {J}. {P}. and {V}oss, {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{R}ecently, the method widely used to determine {N}-15(2) fixation rates in marine and freshwater environments was found to underestimate rates because the dissolution of the added {N}-15(2) gas bubble in seawater takes longer than theoretically calculated. {A}s a solution to the potential underestimate of rate measurements, the usage of the enriched water method was proposed to provide constant {N}-15(2) enrichment. {S}till, the superiority of enriched water method over the previously used bubble injection remains inconclusive. {T}o clarify this issue, we performed laboratory based experiments and implemented the results into an error analysis of {N}-15(2) fixation rates. {M}oreover, we conducted a literature search on the comparison of the two methods to calculate a mean effect size using a meta-analysis approach. {O}ur results indicate that the error potentially introduced by an equilibrium phase of the {N}-15(2) gas is -72% at maximum for experiments with very short incubation times of 1 h. {I}n contrast, the underestimation was negligible for incubations lasting 12-24 h (error is -0.2%). {O}ur meta-analysis indicates that 84% of the measurements in the two groups will overlap and there is a 61% chance that a sample picked at random from the enriched water group will have a higher value than one picked at random from the bubble group. {O}verall, the underestimation of {N}-2 fixation rates when using the bubble method relative to the enriched water method is highly dependent on incubation time and other experimental conditions and cannot be generalized.},
  keywords = {{N}-15(2) fixation ; enriched water method ; bubble method ; diazotrophs ; meta-analysis},
  booktitle = {},
  journal = {{F}rontiers in {M}arine {S}cience},
  volume = {5},
  numero = {},
  pages = {120 [10 ]},
  year = {2018},
  DOI = {10.3389/fmars.2018.00120},
  URL = {https://www.documentation.ird.fr/hor/fdi:010075180},
}