@article{fdi:010075169,
  title = {{P}rotocol for {RYMV} inoculation and resistance evaluation in rice seedlings},
  author = {{G}alzi, {A}gn{\`e}s and {H}{\'e}brard, {E}ug{\'e}nie and {T}raor{\'e}, {O}. and {S}ilu{\'e}, {D}. and {A}lbar, {L}aurence},
  editor = {},
  language = {{ENG}},
  abstract = {{R}ice yellow mottle virus ({RYMV}), a mechanically transmitted virus that causes serious damage to cultivated rice plants, is endemic to {A}frica. {V}arietal selection for resistance is considered to be the most effective and sustainable management strategy. {S}tandardized resistance evaluation procedures are required for the identification and characterization of resistance sources. {T}his paper describes a protocol for mechanical inoculation of rice seedlings with {RYMV} and two methods of resistance evaluation - one based on a symptom severity index and the other on virus detection through double antibody sandwich-enzyme linked immunosorbent assay ({DAS}-{ELISA}).},
  keywords = {{R}ice ; {RYMV} ; {I}noculation ; {R}esistance ; {ELISA} ; {S}ymptoms},
  booktitle = {},
  journal = {{B}io-{P}rotocol},
  volume = {8},
  numero = {11},
  pages = {e2863 [13 p.]},
  year = {2018},
  DOI = {10.21769/{B}io{P}rotoc.2863},
  URL = {https://www.documentation.ird.fr/hor/fdi:010075169},
}