@article{fdi:010074863,
  title = {{D}evelopment of diagnostic {SCAR} markers for {M}eloidogyne graminicola, {M}-oryzae, and {M}-salasi associated with irrigated rice fields in {A}mericas},
  author = {{M}attos, {V}. {D}. and {M}ulet, {K}. and {C}ares, {J}. {E}. and {G}omes, {C}. {B}. and {F}ernandez, {D}iana and de {S}a, {M}. {F}. {G}. and {C}arneiro, {R}. {M}. {D}. {G}. and {C}astagnone-{S}ereno, {P}.},
  editor = {},
  language = {{ENG}},
  abstract = {{R}oot-knot nematodes ({RKN}) cause important production losses of rice ({O}ryza sativa {L}.) in the world. {T}ogether with {M}eloidogyne graminicola {G}olden and {B}irchfield 1965, {M}. oryzae {M}aas, {S}anders and {D}ede, 1978 and {M}. salasi {L}opez, 1984 have been causing damages in irrigated rice fields in {C}entral and {S}outh {A}merica. {I}n addition, six other {RKN} species may occur in rice fields in other regions of the world. {C}orrect identification of {M}eloidogyne spp. is difficult but essential for the management of rice {RKN}s. {T}he objective of this study was to develop some species-specific molecular markers for the diagnosis of {S}outh {A}merican {RKN} rice-related species. {I}sozyme phenotypes indicated the occurrence of some {RKN} species in the {B}razilian samples, namely {M}. graminicola, {M}. oryzae, {M}. javanica, and two cryptic species designated as {M}eloidogyne sp. 2 and {M}eloidogyne sp. 3. {R}andom amplified polymorphic {DNA} ({RAPD}) analysis of 16 isolates revealed interspecific genetic polymorphism between {M}eloidogyne spp., but isolates belonging to the same species (i.e., sharing the same esterase phenotype) always clustered together, whatever the species considered. {S}pecific {SCAR} markers of 230, 120, and 160 bp were developed for {M}. graminicola, {M}. oryzae, and {M}. salasi, respectively. {T}hese {SCAR} markers may be potential molecular tools for application in routine diagnostic procedures subject to their validation with other rice {RKN} field populations in the world.},
  keywords = {{AMERIQUE} {DU} {SUD} ; {BRESIL}},
  booktitle = {},
  journal = {{P}lant {D}isease},
  volume = {103},
  numero = {1},
  pages = {83--88},
  ISSN = {0191-2917},
  year = {2019},
  DOI = {10.1094/pdis-12-17-2015-re},
  URL = {https://www.documentation.ird.fr/hor/fdi:010074863},
}