Tao D. Y., McGill B., Hamerly T., Kobayashi T., Khare P., Dziedzic A., Leski T., Holtz A., Shull B., Jedlicka A. E., Walzer A., Slowey P. D., Slowey C. C., Nsango S. E., Stenger D. A., Chaponda M., Mulenga M., Jacobsen K. H., Sullivan D. J., Ryan S. J., Ansumana R., Moss W. J., Morlais Isabelle, Dinglasan R. R. (2019). A saliva-based rapid test to quantify the infectious subclinical malaria parasite reservoir. Science Translational Medicine, 11 (473), art. eaan4479 [13 p.]. ISSN 1946-6234.
Titre du document
A saliva-based rapid test to quantify the infectious subclinical malaria parasite reservoir
Année de publication
2019
Auteurs
Tao D. Y., McGill B., Hamerly T., Kobayashi T., Khare P., Dziedzic A., Leski T., Holtz A., Shull B., Jedlicka A. E., Walzer A., Slowey P. D., Slowey C. C., Nsango S. E., Stenger D. A., Chaponda M., Mulenga M., Jacobsen K. H., Sullivan D. J., Ryan S. J., Ansumana R., Moss W. J., Morlais Isabelle, Dinglasan R. R.
Source
Science Translational Medicine, 2019,
11 (473), art. eaan4479 [13 p.] ISSN 1946-6234
A large proportion of ongoing malaria parasite transmission is attributed to low-density subclinical infections not readily detected by available rapid diagnostic tests (RDTs) or microscopy. Plasmodium fakiparum gametocyte carriage is subclinical, but gametocytemic individuals comprise the parasite reservoir that leads to infection of mosquitoes and local transmission. Effective detection and quantification of these carriers can help advance malaria elimination strategies. However, no point-of-need (PON) RDTs for gametocyte detection exist, much less one that can perform noninvasive sampling of saliva outside a clinical setting. Here, we report on the discovery of 35 parasite markers from which we selected a single candidate for use in a PON RDT. We performed a cross-sectional, multi-omics study of saliva from 364 children with subclinical infection in Cameroon and Zambia and produced a prototype saliva-based PON lateral flow immunoassay test for P. fakiparum gametocyte carriers. The test is capable of identifying submicroscopic carriage in both clinical and nonclinical settings and is compatible with archived saliva samples.
Plan de classement
Santé : généralités [050]
;
Entomologie médicale / Parasitologie / Virologie [052]
Description Géographique
AFRIQUE SUBSAHARIENNE
Localisation
Fonds IRD [F B010074860]
Identifiant IRD
fdi:010074860
