%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Segeral, O.
%A N'Diaye, D. S.
%A Prak, S.
%A Nouhin, J.
%A Chhun, S.
%A Khamduang, W.
%A Chim, K.
%A Roque-Afonso, A. M.
%A Piola, P.
%A Borand, L.
%A Ngo-Giang-Huong, Nicole
%A Rouet, F.
%A ANRS Study Group
%T Usefulness of a serial algorithm of HBsAg and HBeAg rapid diagnosis tests to detect pregnant women at risk of HBV mother-to-child transmission in Cambodia, the ANRS 12328 pilot study
%D 2018
%L fdi:010074825
%G ENG
%J Journal of Clinical Virology
%@ 1386-6532
%K Cambodia ; Hepatitis B virus ; Rapid diagnostic tests ; Pregnant women ; Mother-to-child transmission
%K CAMBODGE
%M ISI:000453568100006
%P 29-34
%R 10.1016/j.jcv.2018.10.007
%U https://www.documentation.ird.fr/hor/fdi:010074825
%> https://www.documentation.ird.fr/intranet/publi/2019/01/010074825.pdf
%V 109
%W Horizon (IRD)
%X Background: In Cambodia, access to hepatitis B surface antigen (HBsAg) screening is low for pregnant women and Hepatitis B Virus (HBV) DNA quantification is poorly accessible. Objectives: To evaluate the performance of a serial algorithm using two HBV rapid diagnostic tests (RDTs), in which samples positive for HBsAg were further tested for HBeAg as a surrogate marker for HBV DNA quantification. Study design: In 2015, we prospectively collected plasma samples from 250 pregnant women consulting for antenatal care in one hospital in Phnom Penh including 128 with a known positive HBsAg status. All specimens were tested with the SD BIOLINE HBsAg RDT and HBsAg ELISA assay. In ELISA-positive samples, HBeAg status was determined using the SD BIOLINE HBeAg RDT and HBV DNA quantification was assessed. Results: Sensitivity and specificity of HBsAg RDT were 99.2% (97.7-99.9) and 100% (97.0-100), respectively. Among the 128 ELISA-positive samples, 29 (23%) tested HBeAg positive and 34 (26.5%) had HBV DNA > 5.3 Log(10) IU/mL. Sensitivity and specificity of HBeAg RDT in identifying viremic samples were 76.5% (62.2.0-90.7) and 96.8% (93.3-100) for HBV DNA > 5.3 Log(10) IU/mL and 89.3% (77.8-100) and 96.0% (92.2-99.8) for HBV DNA > 7.3 Log(10) IU/mL. Among the 99 negative HBeAg RDT women, 8 had HBV DNA > 5.3 Log10 IU/mL and 7 of them harbored BCP/PC HBV mutants. Conclusions: A combination of HBsAg and HBeAg RDTs could be a low-cost strategy to identify HBV-infected pregnant women at risk of perinatal transmission in a country were HBV DNA quantification is not routinely available.
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