@article{fdi:010074825,
  title = {{U}sefulness of a serial algorithm of {HB}s{A}g and {HB}e{A}g rapid diagnosis tests to detect pregnant women at risk of {HBV} mother-to-child transmission in {C}ambodia, the {ANRS} 12328 pilot study},
  author = {{S}egeral, {O}. and {N}'{D}iaye, {D}. {S}. and {P}rak, {S}. and {N}ouhin, {J}. and {C}hhun, {S}. and {K}hamduang, {W}. and {C}him, {K}. and {R}oque-{A}fonso, {A}. {M}. and {P}iola, {P}. and {B}orand, {L}. and {N}go-{G}iang-{H}uong, {N}icole and {R}ouet, {F}. and {ANRS} {S}tudy {G}roup},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {I}n {C}ambodia, access to hepatitis {B} surface antigen ({HB}s{A}g) screening is low for pregnant women and {H}epatitis {B} {V}irus ({HBV}) {DNA} quantification is poorly accessible. {O}bjectives: {T}o evaluate the performance of a serial algorithm using two {HBV} rapid diagnostic tests ({RDT}s), in which samples positive for {HB}s{A}g were further tested for {HB}e{A}g as a surrogate marker for {HBV} {DNA} quantification. {S}tudy design: {I}n 2015, we prospectively collected plasma samples from 250 pregnant women consulting for antenatal care in one hospital in {P}hnom {P}enh including 128 with a known positive {HB}s{A}g status. {A}ll specimens were tested with the {SD} {BIOLINE} {HB}s{A}g {RDT} and {HB}s{A}g {ELISA} assay. {I}n {ELISA}-positive samples, {HB}e{A}g status was determined using the {SD} {BIOLINE} {HB}e{A}g {RDT} and {HBV} {DNA} quantification was assessed. {R}esults: {S}ensitivity and specificity of {HB}s{A}g {RDT} were 99.2% (97.7-99.9) and 100% (97.0-100), respectively. {A}mong the 128 {ELISA}-positive samples, 29 (23%) tested {HB}e{A}g positive and 34 (26.5%) had {HBV} {DNA} > 5.3 {L}og(10) {IU}/m{L}. {S}ensitivity and specificity of {HB}e{A}g {RDT} in identifying viremic samples were 76.5% (62.2.0-90.7) and 96.8% (93.3-100) for {HBV} {DNA} > 5.3 {L}og(10) {IU}/m{L} and 89.3% (77.8-100) and 96.0% (92.2-99.8) for {HBV} {DNA} > 7.3 {L}og(10) {IU}/m{L}. {A}mong the 99 negative {HB}e{A}g {RDT} women, 8 had {HBV} {DNA} > 5.3 {L}og10 {IU}/m{L} and 7 of them harbored {BCP}/{PC} {HBV} mutants. {C}onclusions: {A} combination of {HB}s{A}g and {HB}e{A}g {RDT}s could be a low-cost strategy to identify {HBV}-infected pregnant women at risk of perinatal transmission in a country were {HBV} {DNA} quantification is not routinely available.},
  keywords = {{C}ambodia ; {H}epatitis {B} virus ; {R}apid diagnostic tests ; {P}regnant women ; {M}other-to-child transmission ; {CAMBODGE}},
  booktitle = {},
  journal = {{J}ournal of {C}linical {V}irology},
  volume = {109},
  numero = {},
  pages = {29--34},
  ISSN = {1386-6532},
  year = {2018},
  DOI = {10.1016/j.jcv.2018.10.007},
  URL = {https://www.documentation.ird.fr/hor/fdi:010074825},
}