@article{fdi:010074814,
  title = {{D}ynamics of bacterial communities mediating the treatment of an as-rich acid mine drainage in a field pilot},
  author = {{L}aroche, {E}. and {C}asiot, {C}. and {F}ernandez-{R}ojo, {L}. and {D}esoeuvre, {A}. and {T}ardy, {V}. and {B}runeel, {O}dile and {B}attaglia-{B}runet, {F}. and {J}oulian, {C}. and {H}ery, {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{P}assive treatment based on iron biological oxidation is a promising strategy for {A}rsenic ({A}s)-rich acid mine drainage ({AMD}) remediation. {I}n the present study, we characterized by 16{S} r{RNA} metabarcoding the bacterial diversity in a field-pilot bioreactor treating extremely {A}s-rich {AMD} in situ, over a 6 months monitoring period. {I}nside the bioreactor, the bacterial communities responsible for iron and arsenic removal formed a biofilm ("biogenic precipitate") whose composition varied in time and space. {T}hese communities evolved from a structure at first similar to the one of the feed water used as an inoculum to a structure quite similar to the natural biofilm developing in situ in the {AMD}. {O}ver the monitoring period, iron-oxidizing bacteria always largely dominated the biogenic precipitate, with distinct populations ({G}allionella, {F}errovum, {L}eptospirillum, {A}cidithiobacillus, {F}erritrophicum), whose relative proportions extensively varied among time and space. {A} spatial structuring was observed inside the trays (arranged in series) composing the bioreactor. {T}his spatial dynamic could be linked to the variation of the physico-chemistry of the {AMD} water between the raw water entering and the treated water exiting the pilot. {A}ccording to redundancy analysis ({RDA}), the following parameters exerted a control on the bacterial communities potentially involved in the water treatment process: dissolved oxygen, temperature, p{H}, dissolved sulfates, arsenic and {F}e({II}) concentrations and redox potential. {A}ppreciable arsenite oxidation occurring in the bioreactor could be linked to the stable presence of two distinct monophylogenetic groups of {T}hiomonas related bacteria. {T}he ubiquity and the physiological diversity of the bacteria identified, as well as the presence of bacteria of biotechnological relevance, suggested that this treatment system could be applied to the treatment of other {AMD}.},
  keywords = {acid mine drainage ; arsenic ; bioremediation ; eco-engineering ; iron-oxidizing bacteria ; arsenic-oxidizing bacteria ; microbial ecotoxicology ; {FRANCE}},
  booktitle = {},
  journal = {{F}rontiers in {M}icrobiology},
  volume = {9},
  numero = {},
  pages = {art. 3169 [13 p.]},
  ISSN = {1664-302{X}},
  year = {2018},
  DOI = {10.3389/fmicb.2018.03169},
  URL = {https://www.documentation.ird.fr/hor/fdi:010074814},
}