@article{fdi:010074749,
  title = {{A} phylogenetic framework of the legume genus {A}eschynomene for comparative genetic analysis of the {N}od-dependent and {N}od-independent symbioses},
  author = {{B}rottier, {L}aurent and {C}haintreuil, {C}l{\'e}mence and {S}imion, {P}. and {S}cornavacca, {C}. and {R}ivallan, {R}. and {M}ournet, {P}. and {M}oulin, {L}ionel and {L}ewis, {G}. {P}. and {F}ardoux, {J}o{\¨e}l and {B}rown, {S}. {C}. and {G}omez-{P}acheco, {M}. and {B}ourges, {M}. and {H}ervouet, {C}. and {G}ueye, {M}. and {D}uponnois, {R}obin and {R}amanankierana, {H}. and {R}andriambanona, {H}. and {V}androt, {H}. and {Z}abaleta, {M}. and {D}as{G}upta, {M}. and {D}'{H}ont, {A}. and {G}iraud, {E}ric and {A}rrighi, {J}ean-{F}ran{\c{c}}ois},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {A}mong semi-aquatic species of the legume genus {A}eschynomene, some have the property of being nodulated by photosynthetic {B}radyrhizobium lacking the nod{ABC} genes necessary for the synthesis of {N}od factors. {K}nowledge of the specificities underlying this {N}od-independent symbiosis has been gained from the model legume {A}eschynomene evenia but our understanding remains limited due to the lack of comparative genetics with related taxa using a {N}od factor-dependent process. {T}o fill this gap, we combined different approaches to perform a thorough comparative analysis in the genus {A}eschynomene. {R}esults: {T}his study significantly broadened previous taxon sampling, including in allied genera, in order to construct a comprehensive phylogeny. {I}n the phylogenetic tree, five main lineages were delineated, including a novel lineage, the {N}od-independent clade and another one containing a polytomy that comprised several {A}eschynomene groups and all the allied genera. {T}his phylogeny was matched with data on chromosome number, genome size and low-copy nuclear gene sequences to reveal the diploid species and a polytomy containing mostly polyploid taxa. {F}or these taxa, a single allopolyploid origin was inferred and the putative parental lineages were identified. {F}inally, nodulation tests with different {B}radyrhizobium strains revealed new nodulation behaviours and the diploid species outside of the {N}od-independent clade were compared for their experimental tractability and genetic diversity. {C}onclusions: {T}he extended knowledge of the genetics and biology of the different lineages sheds new light of the evolutionary history of the genus {A}eschynomene and they provide a solid framework to exploit efficiently the diversity encountered in {A}eschynomene legumes. {N}otably, our backbone tree contains all the species that are diploid and it clarifies the genetic relationships between the {N}od-independent clade and the {N}od-dependent lineages. {T}his study enabled the identification of {A}. americana and {A}. patula as the most suitable species to undertake a comparative genetic study of the {N}od-independent and {N}od-dependent symbioses.},
  keywords = {{A}eschynomene ; {G}enetics ; {L}egumes ; {N}odulation ; {P}hylogenetics ; {P}olyploidy ; {S}ymbiosis},
  booktitle = {},
  journal = {{BMC} {P}lant {B}iology},
  volume = {18},
  numero = {},
  pages = {art. 333 [15 p.]},
  ISSN = {1471-2229},
  year = {2018},
  DOI = {10.1186/s12870-018-1567-z},
  URL = {https://www.documentation.ird.fr/hor/fdi:010074749},
}