@article{fdi:010074117,
  title = {{P}rofiling of {H}ura crepitans {L}. latex by ultra-high-performance liquid chromatography/atmospheric pressure chemical ionisation linear ion trap {O}rbitrap mass spectrometry},
  author = {{T}rinel, {M}. and {J}ullian, {V}al{\'e}rie and {L}e {L}amer, {A}. {C}. and {M}hamdi, {I}. and {M}ejia, {K}. and {C}astillo, {D}. and {C}abanillas, {B}. {J}. and {F}abre, {N}.},
  editor = {},
  language = {{ENG}},
  abstract = {{I}ntroduction: {T}he phytochemistry of the latex of {H}ura crepitans {L}. ({E}uphorbiaceae), a widespread tree in the {A}mazonian forest having many uses, is little known. {O}nly huratoxin, a daphnane diterpene orthoester, has been described despite the high pharmacological potential of this kind of compounds. {G}lucosphingolipids (cerebrosides) are also known to be distributed in {E}uphorbiaceae latexes. {O}bjective: {T}o tentatively identify daphnanes diterpenes and cerebrosides in the latex of {H}. crepitans. {M}ethods: {A}n ethanolic extract of the lyophilised latex of {H}. crepitans was analysed by ultra-high-performance liquid chromatography ({UHPLC}) coupled with positive and negative atmospheric pressure chemical ionisation high-resolution mass spectrometry ({APCI}-{HRMS}) method using a quadrupole/linear ion trap/{O}rbitrap ({LTQ}-{O}rbitrap). {T}andem mass spectrometry ({MS}/{MS}) spectra were recorded by two different fragmentation modes: collision induced dissociation ({CID}) and higher-energy collisional dissociation ({HCD}). {R}esults: {T}he analysis of {CID}- and {HCD}-{MS}/{MS} spectra allowed to propose fragmentation patterns for daphnane esters and cerebrosides and highlight diagnostic ions in positive and negative ion modes. {A} total of 34 compounds including 24 daphnane esters and 10 cerebrosides have been tentatively annotated. {A}mong them, 17 daphnane diterpenes bearing one or two acyl chains are new compounds and the cerebrosides are described in the genus {H}ura for the first time. {C}onclusion: {T}his study revealed the chemical constituents of the latex of {H}. crepitans and particularly its richness and chemical diversity in daphnane diterpenes, more frequently encountered in the species of {T}hymelaeaceae.},
  keywords = {cerebrosides ; daphnane diterpenes ; fragmentation patterns ; {H}ura crepitans ; {UHPLC}-{MS}/{MS} ; {PEROU} ; {AMAZONIE}},
  booktitle = {},
  journal = {{P}hytochemical {A}nalysis},
  volume = {29},
  numero = {6},
  pages = {627--638},
  ISSN = {0958-0344},
  year = {2018},
  DOI = {10.1002/pca.2776},
  URL = {https://www.documentation.ird.fr/hor/fdi:010074117},
}