@article{fdi:010074064,
  title = {{M}etabarcoding by capture using a single {COI} probe ({MCSP}) to identify and quantify fish species in ichthyoplankton swarms},
  author = {{M}ariac, {C}{\'e}dric and {V}igouroux, {Y}ves and {D}uponchelle, {F}abrice and {G}arcia-{D}avila, {C}. and {N}unez {R}odriguez, {J}esus and {D}esmarais, {E}. and {R}enno, {J}ean-{F}ran{\c{c}}ois},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he ability to determine the composition and relative frequencies of fish species in large ichthyoplankton swarms could have extremely important ecological applications {H}owever, this task is currently hampered by methodological limitations. {W}e proposed a new method for {A}mazonian species based on hybridization capture of the {COI} gene {DNA} from a distant species ({D}anio rerio), absent from our study area (the {A}mazon basin). {T}he {COI} sequence of this species is approximately equidistant from all {COI} of {A}mazonian species available. {B}y using this sequence as probe we successfully facilitated the simultaneous identification of fish larvae belonging to the order {S}iluriformes and to the {C}haraciformes represented in our ichthyoplankton samples. {S}pecies relative frequencies, estimated by the number of reads, showed almost perfect correlations with true frequencies estimated by a {S}anger approach, allowing the development of a quantitative approach. {W}e also proposed a further improvement to a previous protocol, which enables lowering the sequencing effort by 40 times. {T}his new {M}etabarcoding by {C}apture using a {S}ingle {P}robe ({MCSP}) methodology could have important implications for ecology, fisheries management and conservation in fish biodiversity hotspots worldwide. {O}ur approach could easily be extended to other plant and animal taxa.},
  keywords = {{AMERIQUE} {DU} {SUD} ; {AMAZONE} {BASSIN}},
  booktitle = {},
  journal = {{P}los {O}ne},
  volume = {13},
  numero = {9},
  pages = {e0202976 [15 p.]},
  ISSN = {1932-6203},
  year = {2018},
  DOI = {10.1371/journal.pone.0202976},
  URL = {https://www.documentation.ird.fr/hor/fdi:010074064},
}