@article{fdi:010074056,
  title = {{M}ixotrophic metabolism by natural communities of unicellular cyanobacteria in the western tropical {S}outh {P}acific {O}cean},
  author = {{D}uhamel, {S}. and {V}an {W}ambeke, {F}. and {L}efevre, {D}. and {B}enavides, {M}ar and {B}onnet, {S}ophie},
  editor = {},
  language = {{ENG}},
  abstract = {{C}yanobacteria are major contributors to ocean biogeochemical cycling. {H}owever, mixotrophic metabolism and the relative importance of inorganic and organic carbon assimilation within the most abundant cyanobacteria are still poorly understood. {W}e explore the ability of {P}rochlorococcus and {S}ynechococcus to assimilate organic molecules with variable {C}:{N}:{P} composition and its modulation by light availability and photosynthetic impairment. {W}e used a combination of radiolabelled molecules incubations with flow cytometry cell sorting to separate picoplankton groups from the western tropical {S}outh {P}acific {O}cean. {P}rochlorococcus and {S}ynechococcus assimilated glucose, leucine and {ATP} at all stations, but cell-specific assimilation rates of {N} and {P} containing molecules were significantly higher than glucose. {I}ncubations in the dark or with an inhibitor of photosystem {II} resulted in reduced assimilation rates. {L}ight-enhanced cell-specific glucose uptake was generally higher for cyanobacteria (approximate to 50%) than for the low nucleic acid fraction of bacterioplankton ({LNA}, approximate to 35%). {O}ur results confirm previous findings, based mainly on cultures and genomic potentials, showing that {P}rochlorococcus and {S}ynechococcus have a flexible mixotrophic metabolism, but demonstrate that natural populations remain primarily photoautotrophs. {O}ur findings indicate that mixotrophy by marine cyanobacteria is more likely to be an adaptation to low inorganic nutrient availability rather than a facultative pathway for carbon acquisition.},
  keywords = {{PACIFIQUE} {SUD}},
  booktitle = {},
  journal = {{E}nvironmental {M}icrobiology},
  volume = {20},
  numero = {8},
  pages = {2743--2756},
  ISSN = {1462-2912},
  year = {2018},
  DOI = {10.1111/1462-2920.14111},
  URL = {https://www.documentation.ird.fr/hor/fdi:010074056},
}