Salmi A., Boulila F., Bourebaba Y., Le Roux C., Belhadi D., Lajudie Philippe de. (2018). Phylogenetic diversity of Bradyrhizobium strains nodulating Calicotome spinosa in the Northeast of Algeria. Systematic and Applied Microbiology, 41 (5), p. 452-459. ISSN 0723-2020.
Titre du document
Phylogenetic diversity of Bradyrhizobium strains nodulating Calicotome spinosa in the Northeast of Algeria
Salmi A., Boulila F., Bourebaba Y., Le Roux C., Belhadi D., Lajudie Philippe de
Source
Systematic and Applied Microbiology, 2018,
41 (5), p. 452-459 ISSN 0723-2020
Fifty-two slow-growing strains were isolated from root nodules of Calico tome spinosa grown in the Northeast of Algeria and grouped in 24 rep-PCR clusters. One representative strain for each profile was further phylogenetically characterized. The nearly complete 16S rRNA gene sequence indicated that all strains were affiliated to Bradyrhizobium. Multi-Locus Sequence Analysis (MLSA) of the atpD, glnII and recA genes and of the 16S-23S rRNA internal transcribed spacer (ITS) showed that these strains formed four divergent clusters: one close to Bradyrhizobium canariense and Bradyrhizobium lupini and three others separate from all the described species, representing three putative new Bradyrhizobium species. A phylogenetic analysis based on the nodC gene sequence affiliated the strains to either of the two symbiovars, genistearum or retamae.
